Adiponectin regulates ACTH secretion and the HPAA in an AMPK-dependent manner in pituitary corticotroph cells
Adiponectin regulates ACTH secretion and the HPAA in an AMPK-dependent manner in pituitary corticotroph cells
复制标题
脂联素以 AMPK 依赖性方式调节垂体促肾上腺皮质细胞中 ACTH 的分泌和 HPAA
DOI：
10.1016/j.mce.2013.12.007
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发表时间：
2014-03-05
影响因子：
4.1
通讯作者：
Ma, Qinyun
中科院分区：
文献类型：
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作者：
Chen, Maopei;Wang, Zhiquan;Ma, Qinyun
已知脂肪因子可以调节下丘脑-垂体-肾上腺轴（HPAA）。本研究证实脂联素通过影响垂体促皮质细胞调节HPAA。利用RT-PCR和免疫荧光技术，我们确定了脂联素受体在垂体促皮质肿瘤细胞（at -20细胞和人促皮质肿瘤）中表达。脂联素刺激钙内流，增加基底ACTH分泌，而不影响促肾上腺皮质激素释放激素（CRH）刺激的ACTH分泌，这很可能是由于脂联素表达抑制CRH受体1 （CRHR1）所致。脂联素也能急性刺激原代培养垂体细胞ACTH的释放。最后，脂联素在at -20细胞中直接磷酸化5' amp活化蛋白激酶（AMPK）。脂联素的作用被AICAR模拟，而AICAR被化合物c阻断。综上所述，我们的研究结果表明，脂联素刺激垂体促皮质细胞ACTH分泌并下调CRHR1可能是通过ampk依赖的机制。2013爱思唯尔爱尔兰有限公司版权所有。
It is known that adipokines can regulate the hypothalamic-pituitary-adrenal axis (HPAA). In this study, we confirmed that adiponectin regulates the HPAA by affecting pituitary corticotroph cells. Using RT-PCR and immunofluorescence, we determined that adiponectin receptors were expressed in pituitary corticotroph tumour cells (AtT-20 cells and human corticotroph tumours). Adiponectin stimulated calcium influx and increased basal ACTH secretion without affecting corticotrophin-releasing hormone (CRH)-timulated ACTH secretion, which was most likely due to the expression of adiponectin repressing CRH receptor 1 (CRHR1). Adiponectin also acutely stimulated ACTH release in primary culture pituitary cells. Lastly, adiponectin directly phosphorylated 5' AMP-activated protein kinase (AMPK) in AtT-20 cells. The effects of adiponectin were mimicked by AICAR, which was blocked by compound C. Taken together, our results suggested that adiponectin stimulated ACTH secretion and down-regulated CRHR1 possibly via an AMPK-dependent mechanism in pituitary corticotroph cells. (C) 2013 Elsevier Ireland Ltd. All rights reserved.