Expression of macrophage migration inhibitory factor in rat retina and its immunohistochemical localization
Expression of macrophage migration inhibitory factor in rat retina and its immunohistochemical localization
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DOI：
10.1016/s0165-5728(97)00061-1
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发表时间：
1997-07-01
影响因子：
3.3
通讯作者：
Nishihira, J
中科院分区：
文献类型：
--
作者：
Matsuda, A;Tagawa, Y;Nishihira, J
巨噬细胞移动抑制因子（Macrophage migration inhibitory factor，MIF）在有丝分裂刺激或抗原刺激诱导的T细胞活化中起重要的调节作用。我们检测了MIF在大鼠视网膜中的表达。视网膜组织匀浆的逆转录-聚合酶链反应分析显示，MIF mRNA的组成型表达。Western blot分析也观察到MIF蛋白的单一特异性条带。免疫组化显示，MIF定位于星形胶质细胞，Muller细胞和色素上皮（RPE）细胞与抗大鼠MIF抗体反应的视网膜组织的冰冻切片。这一拓扑发现证实了共定位的MIF蛋白与胶质细胞酸性蛋白和波形蛋白，这是假定的免疫组织化学标记的星形胶质细胞和Muller sells。众所周知，视网膜神经胶质细胞以及RPE细胞在视网膜中的炎症和免疫反应中起着积极的作用。考虑到这些事实，MIF在这些细胞中的组成性表达表明该蛋白有助于调节视网膜组织炎症及其局部免疫的可能性。
Macrophage migration inhibitory factor (MIF) plays an important regulatory role for the T-cell activation induced by mitogenic or antigenic stimuli. We examined expression of MIF in rat retina. Reverse transcription-polymerase chain reaction analysis of a retinal tissue homogenate revealed that MIF mRNA was constitutively expressed. A single band specific for MIF protein was also observed by Western blot analysis. Immunohistochemistry using frozen sections of retinal tissues reacted with an anti-rat MIF antibody showed that MIF was localized in astrocytes, Muller cells and pigment epithelial (RPE) cells. This topological finding was confirmed by colocalization of MIF protein with glial fibrillary acidic protein and vimentin, which are putative immunohistochemical markers for astrocytes and Muller sells. It is well known that the retinal glial cells, as well as RPE cells, play an active role in inflammatory and immunological responses in the retina. Considering these facts, constitutive expression of MIF in these cells suggested the possibility that the protein contributes to regulation of retinal tissue inflammation as well as its local immunity.