Expression patterns of claudin family of tight junction membrane proteins in developing mouse submandibular gland
Expression patterns of claudin family of tight junction membrane proteins in developing mouse submandibular gland
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DOI：
10.1002/dvdy.20142
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发表时间：
2004-10-01
影响因子：
2.5
通讯作者：
Hieda, Y
中科院分区：
文献类型：
--
作者：
Hashizume, A;Ueno, T;Hieda, Y
在这里，我们研究了紧密连接跨膜蛋白家族在发育中的小鼠下颌下腺的表达。通过逆转录聚合酶链反应、蛋白质印迹和免疫荧光显微镜获得的数据显示紧密连接蛋白-3至-8、-10和-11在上皮紧密连接处的表达和定位。对取自胚胎（E）14、E16和新生小鼠的腺体的检查揭示了这些claudin在发育中的上皮中的差异表达模式。紧密连接蛋白-3、-5和-7在所有发育阶段的导管的所有管腔上皮细胞中表达，并且在E16和以后的终末小管的管腔上皮细胞中表达。Claudin-4主要表达于各个发育阶段的导管中。claudin-6和-8的表达也仅限于在E14和E16的导管，但出生后，前者是不可检测的，而后者表达在导管和终末小管。Claudin-10和Claudin-11在E16及以后主要在终末小管中可检测到。除了在上皮细胞中发现外，claudin-5还在某些间充质细胞中表达，可能是内皮细胞。这些结果将为进一步研究小管发生和紧密连接的生理调控提供有价值的资源。(C)2004 Wiley-Liss，Inc.
Here, we investigated the expression of the claudin family of tight junction transmembrane proteins in the developing mouse submandibular gland. Data obtained by reverse transcriptase-polymerase chain reaction, Western blot, and immunofluorescence microscopy showed the expression and localization of claudin-3 to -8, -10, and -11 at epithelial tight junctions. Examination of the glands taken from embryonic day (E) 14, E16, and newborn mice revealed differential expression patterns of these claudins in the developing epithelium. Claudin-3, -5, and -7 were expressed in all of the luminal epithelial cells of the ducts at all of the developmental stages examined and in those of terminal tubules at El 6 and later. Claudin-4 was expressed mainly in the ducts at all the developmental stages. The expression of claudin-6 and -8 was also restricted to the ducts at E14 and E16; but after birth, the former was undetectable, whereas the latter was expressed in both the ducts and terminal tubules. Claudin-10 and -11 were detectable mainly in the terminal tubules at E16 and later. In addition to being found in the epithelium, claudin-5 was also expressed in certain mesenchymal cells, probably endothelial cells. These results will provide a valuable resource for further investigation of tubulogenesis and physiological regulation of claudin-based tight junctions. (C) 2004 Wiley-Liss, Inc.