A 3D model of Reelin subrepeat regions predicts Reelin binding to carbohydrates.
A 3D model of Reelin subrepeat regions predicts Reelin binding to carbohydrates.
复制标题
Reelin 亚重复区域的 3D 模型预测 Reelin 与碳水化合物的结合。
DOI：
10.1016/j.brainres.2006.07.128
复制
发表时间：
2006
期刊：
影响因子：
2.9
通讯作者：
Keller,Flavio
中科院分区：
文献类型：
--
作者：
Panteri,Roger;Paiardini,Alessandro;Keller,Flavio
Reelin是细胞外基质（ECM）的大分子，其在脊椎动物物种的皮质发育的早期阶段调节神经元定位。Reelin分子可以细分为一个较小的N-末端结构域，显示与F-spondin同源，和一个较大的C-末端区域，含有8个EGF样重复。Reelin在ECM中的定位，其大尺寸和其一级结构的模块化组织使我们假设其模块的结构类似于ECM蛋白质如聚集蛋白、层粘连蛋白和血小板反应蛋白中常见的结构域。因此，我们进行了序列比对和分子模拟分析，研究的三维折叠的Reelin亚重复区。我们的分析产生了一个初步的模型的核心区域的Reelin亚重复序列，并建议存在于这个三维模型的结构特征常见的多糖结合模块，这是经常发现的ECM的蛋白聚糖。这些发现提供了一个概念框架，进一步的实验，旨在测试的EGF样重复区域的Reelin的功能。
Reelin is a large molecule of the extracellular matrix (ECM) which regulates neuronal positioning during the early stages of cortical development in vertebrate species. The Reelin molecule can be subdivided into a smaller N-terminal domain, showing homology with F-spondin, and a larger C-terminal region containing 8 EGF-like repeats. The localization of Reelin in the ECM, its large dimensions and the modular organization of its primary structure led us to suppose a structure of its modules similar to domains commonly found in ECM proteins such as Agrin, laminins and thrombospondins. We therefore performed a sequence alignment and molecular modeling analysis to study the three-dimensional fold of the Reelin subrepeat regions. Our analysis produces a tentative model of the core region of the Reelin subrepeat sequences and suggests the presence in this 3D model of structural features common to polysaccharide-binding modules which are often found on proteoglycans of the ECM. These findings provide a conceptual framework for further experiments aimed at testing the functions of the EGF-like repeat regions of Reelin.