Antioxidant effects of a dietary supplement: Reduction of indices of oxidative stress in normal subjects by water-soluble chitosan
Antioxidant effects of a dietary supplement: Reduction of indices of oxidative stress in normal subjects by water-soluble chitosan
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DOI：
10.1016/j.fct.2008.10.015
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Tomida, Hisao
中科院分区：
文献类型：
--
作者：
Anraku, Makoto;Fujii, Takeshi;Tomida, Hisao
本文研究了水溶性壳聚糖（一种从几丁质中提取的天然聚合物）对正常志愿者氧化应激指标的影响。壳聚糖治疗4周后，血糖水平、动脉粥样硬化指数显著降低，高密度脂蛋白胆固醇（HDL）升高。壳聚糖处理降低了氧化白蛋白与还原白蛋白的比例，提高了血浆总抗氧化活性（TPA）。TPA与氧化白蛋白比值有良好的相关性。结果表明，氧化白蛋白比率是氧化应激的一个潜在有用的标志物。在体外研究中，与对照组相比，壳聚糖存在时，白蛋白羰基和氢过氧化物呈时间依赖性显著降低（p < 0.05）。壳聚糖还以剂量和时间依赖的方式减少了两种稳定的自由基。结果表明，壳聚糖通过降低体内和体外氧化应激指标，在体循环中具有直接的抗氧化活性。这可能会带来额外的好处，减少血浆碳水化合物和增加高密度脂蛋白水平。它还可以抑制血透患者血清白蛋白的氧化，从而减少与尿毒症相关的氧化应激。(C) 2008 Elsevier Ltd版权所有。
The effect of water-soluble chitosan, a natural polymer derived from chitin, on indices of oxidative stress was investigated in normal volunteers. Treatment with chitosan for 4 weeks produced a significant decrease in levels of plasma glucose, atherogenic index and led to increase in high density lipoprotein cholesterol (HDL). Chitosan treatment also lowered the ratio of oxidized to reduced albumin and increased total plasma antioxidant activity (TPA). There was good correlation between TPA and oxidized albumin ratio. The results indicate that oxidized albumin ratio represents a potentially useful marker of oxidative stress. In in vitro studies, albumin carbonyls and hydroperoxides were significantly decreased in a time-dependent manner in the presence of chitosan, compared with controls (p < 0.05). Chitosan also reduced two stable radicals in a dose- and time-dependent manner. The results suggest that chitosan has a direct antioxidant activity in systemic circulation by lowering the indices of oxidative stress in both in vitro and in vivo studies. This may confer benefits additional to the reduction in plasma carbohydrate and increase in HDL levels. It may also inhibit oxidation of serum albumin commonly observed in patients undergoing hemodialysis, resulting in reduction of oxidative stress associated with uremia. (C) 2008 Elsevier Ltd. All rights reserved.