Toward a better understanding of preeclampsia: Comparative proteomic analysis of preeclamptic placentas
Toward a better understanding of preeclampsia: Comparative proteomic analysis of preeclamptic placentas
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DOI：
10.1002/prca.200700034
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发表时间：
2007-12-01
影响因子：
2
通讯作者：
Han, Jin
中科院分区：
文献类型：
--
作者：
Kim, Young Nam;Kim, Hyoung Kyu;Han, Jin
先兆子痫（PE）是一种妊娠特异性高血压、蛋白尿和其他全身性疾病的综合征，是继发于胎盘形成缺陷的广泛内皮功能障碍。从形态学上看，目前的数据显示先兆子痫胎盘的线粒体和绒毛滋养层细胞发生退行性和凋亡性变化。为了揭示胎盘蛋白的叠加改变，可能解释PE的病理生理学，我们进行了2-DE MALDI-TOF MS/MS蛋白质组学分析的差异表达的胎盘蛋白与胎盘8正常和8先兆子痫妊娠。通过Western blot分析确认鉴定的蛋白。我们还进行了形态学评价先兆子痫胎盘在电子和光学显微镜下。结果显示，伴侣蛋白60、GST、VDAC、ERp 29和组织蛋白酶D在PE中显著过表达。这些蛋白质组学研究结果清楚地表明，PE期间可能存在细胞对抗由细胞氧化应激引起的氧化应激的斗争，最终导致恢复或凋亡。这些结果提供了一个更好的理解蛋白质组学的改变，并可能有助于澄清压力相关的变化，先兆子痫胎盘。
Preedampsia (PE), a pregnancy-specific syndrome of hypertension, proteinuria, and other systemic disturbances, is a state of widespread endothelial dysfunction secondary to defective placentation. Morphologically, the current data displayed degenerative and apoptotic changes in the mitochondria and villous trophoblasts of preeclamptic placenta. To reveal the superimposing alterations in placental proteins that might explain the pathophysiology of PE, we performed 2-DE MALDI-TOF MS/MS proteomics analysis of differentially expressed placental proteins with placenta from eight normal and eight preeclamptic pregnancies. The identified proteins were confirmed by Western blot analysis. We also performed morphologic evaluation of preeclamptic placentas under both electron and light microscopy. The results disclosed the marked overexpression of chaperonin 60, GST, VDAC, ERp29, and cathepsin D in PE. These proteomics findings dearly suggest the possible cellular battle against mitochondria-originated oxidative stress during PE that either end up with recovery or apoptosis. These results provide a better understanding of proteomic alterations and may help in clarification of stress-related changes in preeclamptic placentas.