Adiponectin is expressed in the brown adipose tissue and surrounding immature tissues in mouse embryos
Adiponectin is expressed in the brown adipose tissue and surrounding immature tissues in mouse embryos
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DOI：
10.1016/j.bbaexp.2005.06.013
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发表时间：
2005-10-15
期刊：
影响因子：
--
通讯作者：
Yoshioka, H
中科院分区：
文献类型：
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作者：
Fujimoto, N;Matsuo, N;Yoshioka, H
脂联素是脂肪细胞因子之一，是脂肪特异性分泌因子。我们研究了它在胚胎发生过程中的表达。脂联素的转录本在胚胎发生的晚期阶段在胚胎（E）第E16.5天检测到。原位杂交结果表明，脂联素转录本定位于棕色脂肪组织（BAT）和周围的未成熟组织在小鼠胚胎。免疫组化显示脂联素的分布与其mRNA的分布密切相关。在E16.5天血清中也检测到脂联素，其浓度在出生时达到峰值。相比之下，脂联素受体I和2的转录本在E12.5天已经在许多组织中表达。因此，它们的表达谱不同于脂联素本身。此外，使用棕色脂肪细胞的原代培养物的实验表明，脂联素在棕色脂肪细胞中受到各种调节剂的调节，类似于其在白色脂肪组织（WATS）中的调节。提示脂联素在围产期糖脂代谢中具有重要作用。(c)2005 Elsevier B.V.保留所有权利。
Adiponectin is one of the adipocytokines, which are adipose-specific secretory factors. We examined its expression during embryogenesis. Transcripts of adiponectin were detected at a late stage of embryogenesis on embryonic (E) day E16.5. In situ hybridization showed that adiponectin transcripts were localized in brown adipose tissues (BATs) and surrounding immature tissues in mouse embryos. Immunohistochemistry using a specific anti-adiponectin antibody showed that the distribution of adiponectin closely parallels that of its mRNA. Adiponectin was also detected in serum at day E16.5, and its concentration peaked at birth. By contrast, transcripts of both the adiponectin receptors I and 2 were already expressed by day E12.5 in many tissues. Thus, their expression profile differed from that of adiponectin itself. Furthermore, experiments using primary cultures of brown adipocytes showed that adiponectin is regulated in brown adipocytes by various modulators, similar to its regulation in white adipose tissues (WATs). These data indicate that adiponectin has important roles in glucose and lipid metabolism during the perinatal period. (c) 2005 Elsevier B.V. All rights reserved.