Detailed Process of Bone Remodeling After Achievement of Osseointegration in a Rat Implantation Model
Detailed Process of Bone Remodeling After Achievement of Osseointegration in a Rat Implantation Model
复制标题
DOI：
10.1002/ar.20748
复制
发表时间：
2009-01-01
影响因子：
2
通讯作者：
Maeda, Takeyasu
中科院分区：
文献类型：
--
作者：
Haga, Maiko;Fujii, Noritaka;Maeda, Takeyasu
骨整合被认为是牙种植中最合适的种植体-骨界面。然而，即使在骨整合完成后，由种植体腔准备驱动的具有空骨细胞腔隙的受损骨仍然存在。尽管之前的研究表明种植体周围发生了骨重塑，但有关其详细过程的信息却很少。我们的研究旨在利用大鼠上颌骨动物模型建立骨整合后检查钛种植体周围骨的命运。将钛植入物插入大鼠上颌骨的准备好的骨腔中。通过碱性磷酸酶和抗酒石酸酸性磷酸酶的双重染色、骨基质蛋白的免疫组织化学、钙黄绿素活体染色以及电子探针微量分析仪的元素图谱来评估骨形成和成熟过程。 1个月后，在完整的先前存在的骨和新形成的编织骨之间仍保留有空骨细胞腔隙或固缩的骨。 3个月后，通过碱性磷酸酶阳性成骨细胞和酒石酸抗性酸性磷酸酶反应性破骨细胞同步协调的主动骨重塑，其逐渐减少直至完全消失，增厚并被致密骨取代。动态标记在整个实验期间在植入物周围新形成的骨中显示出两条清晰的线。电子探针微量分析仪分析表明，2.5 个月后，新形成的骨骼中 Ca 和 P 的水平随时间顺序增加，与周围骨骼中的 Ca 和 P 水平相同。这些发现表明，实现骨整合后的持续骨重塑会导致致密骨替代受损骨，并改善骨质量。 Anat Rec，292:38-47，2009。(c) 2008 Wiley-Liss, Inc.
Osseointegration is regarded as the most appropriate implant-bone interface in dental implantation. However, damaged bone with empty osteocytic lacunae driven by implant cavity preparation remains even after the completion of osseointegration. Although previous studies have suggested the occurrence of bone remodeling around implants, information on its detailed process is meager. Our study aimed to examine the fate of bone around titanium implants after the establishment of osseointegration on an animal model using the rat maxilla. Titanium implants were inserted into prepared bone cavities of the rat maxilla. Bone formation and maturation processes were evaluated by double staining for alkaline phosphatase and tartrate-resistant acid phosphatase, immunohistochemistry for bone matrix proteins, vital staining with calcein, and elemental mapping with an electron probe microanalyzer. Bone with empty osteocytic lacunae or pyknosis remained between the intact preexisting and newly formed woven bones at post 1 month. It gradually decreased to disappear completely by active bone remodeling with a synchronized coordination of alkaline phosphatase-positive osteoblasts and tartrate-resistant acid phosphatase-reactive osteoclasts at post 3 months, thickening to be replaced by compact bone. Dynamic labeling showed two clear lines in the newly formed bone around the implant through this experimental period. Electron probe microanalyzer analysis demonstrated chronologically increased levels of Ca and P in the newly formed bone identical to those in the surrounding bone at post 2.5 months. These findings indicate that continuous bone remodeling after the achievement of osseointegration causes replacement of the damaged bone by compact bone as well as an improvement in bone quality. Anat Rec,292:38-47, 2009. (c) 2008 Wiley-Liss, Inc.