Karyotype analysis of the B16 mouse melanoma with reassessment of the normal mouse idiogram.
Karyotype analysis of the B16 mouse melanoma with reassessment of the normal mouse idiogram.
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B16 小鼠黑色素瘤的核型分析并重新评估正常小鼠的表型。
DOI：
10.1093/jnci/45.4.789
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
N. Stephenson
中科院分区：
文献类型：
--
作者：
E. M. Stephenson;N. Stephenson
详细分析了B16小鼠黑色素瘤体外原代和17代细胞的核型，并与正常成年雄性C57BL小鼠细胞进行了比较。肿瘤细胞中持续存在两个大的、双臂的、稳中心的标记物，具有个体的显著特征。与正常小鼠相比，最长的远心染色体异常拉长。在41的茎系核型中加入了1分钟。在原代体外培养中，相当多的细胞含有40条染色体，显然缺乏空闲时间。17代培养的细胞，虽然保持了典型的茎系数量，但表现出额外一分钟的合并和多倍体的不同趋势。肿瘤细胞中只有一条可识别的“兔耳”（R.E.）染色体，但在最长的定中心染色体（可能是同工染色体）的每条臂上都有明显的二次收缩，这表明正常小鼠R.E.补体的一个成员可能参与了该标记的形成。为了解释先前发表的小鼠独特型图之间的明显差异，使用了两种不同的计算方法来制备正常C57BL核型的独特型图。对制备B16茎系图的方法进行了说明和比较。
Karyotypes ofin vitroprimary and seventeenth passage cells of the B16 mouse melanoma were analyzed in detail and compared with those of normal adult male C57BL mouse cells. Two large, biarmed, metacentric markers, with individual distinguishing characteristics, were constantly present in the tumor cells. The longest telocentric chromosome was abnormally elongated compared to its counterpart in a normal mouse. A single minute was incorporated in the stemline karyotype of 41. In primaryin vitrocultures, a relatively large number of cells contained 40 chromosomes and apparently lacked a free minute. Cells of seventeenth passage cultures, though maintaining the typical stemline number, showed separate trends toward the incorporation of an additional minute and toward polypoidy. Only one regularly identifiable “rabbit ear” (R.E.) chromosome occurred in the tumor cells, but an obvious secondary constriction in each arm of the longest metacentric, a probable isochromosome, suggested that a member of the normal mouse R.E. complement may have been involved in the formation of this marker. In an attempt to explain apparent discrepancies between previously published mouse idiograms, two different methods of calculation were used to prepare idiograms of normal C57BL karyotypes. Methods for preparation of a B16 stemline idiogram are illustrated and compared.