Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE-UHPLC-MS/MS
Simultaneous determination of 29 pharmaceuticals in fish muscle and plasma by ultrasonic extraction followed by SPE-UHPLC-MS/MS
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超声提取 SPE-UHPLC-MS/MS 同时测定鱼肌肉和血浆中的 29 种药物
DOI：
10.1002/jssc.201701360
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发表时间：
2018
影响因子：
3.1
通讯作者：
Yin Da qiang
中科院分区：
文献类型：
--
作者：
Li You yu;Hu Xia lin;Bao Yi fan;Yin Da qiang
建立了固相萃取-超高效液相色谱-串联质谱法同时检测鱼肉和血浆中29种药物的确证方法。鱼样品用甲醇提取，并使用Oasis HLB固相萃取柱一步富集。采用内标法对29种目标药物进行定量，校准曲线在较宽范围内呈良好线性，决定系数大于0.913。方法的检出限为0.01 ~ 2.00 μg/kg（μg/L）。通过精密度和回收率实验验证了该方法的适用性。29种药物的平均回收率在61 ~ 111%之间，相对标准偏差均小于25%。该方法灵敏、简便、快速、可靠，可用于鱼肉和血浆中29种药物的同时测定。真实的样品测定结果表明，在29种化合物中，鱼肉和血浆中分别检出25种和9种。
A confirmatory method for the simultaneous detection of 29 pharmaceuticals in fish muscle and plasma was developed by using solid‐phase extraction combined with ultra‐high performance liquid chromatography and tandem mass spectrometry. Fish samples were extracted with methanol and enriched using Oasis HLB solid‐phase extraction columns in one step. Twenty‐nine target pharmaceuticals were quantified by the internal standard method and the calibration curves showed good linearity in a wide range with determination coefficients of greater than 0.913. The detection limits of the pharmaceuticals ranged from 0.01 to 2.00 μg/kg (μg/L). The applicability of the method was checked by precision and recovery experiments. The average recoveries of the 29 pharmaceuticals were between 61 and 111%, and all the relative standard deviations were below 25%. Our reported method has been demonstrated to be sensitive, convenient, rapid, and reliable for the simultaneous determination of 29 pharmaceuticals in fish muscle and plasma. Real sample determination showed that 25 and 9 of the 29 compounds were detected in fish muscle and plasma, respectively.