SIMULTANEOUS PREPARATION OF MONONUCLEAR AND POLYMORPHONUCLEAR LEUKOCYTES FROM HORSE BLOOD ON FICOLL-HYPAQUE MEDIUM
SIMULTANEOUS PREPARATION OF MONONUCLEAR AND POLYMORPHONUCLEAR LEUKOCYTES FROM HORSE BLOOD ON FICOLL-HYPAQUE MEDIUM
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DOI：
10.1016/0022-1759(80)90100-3
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发表时间：
1980-01-01
影响因子：
2.2
通讯作者：
THONG, YH
中科院分区：
文献类型：
--
作者：
FERRANTE, A;THONG, YH
高纯度的单核（MN）和多态核（PMN）白细胞可以从马血液中获得，方法类似于先前描述的从人血液中分离这些白细胞的方法。这包括在密度为1.095 g/ml的Ficoll-Hypaque培养基上对马血进行离心。该过程大约需要1小时完成，同时制备MN（纯度为> 98%）和PMN（纯度为> 96%）白细胞。细胞存活率超过95%，细胞保持免疫功能。
Highly purified population of mononuclear (MN) and polymorphonuclear (PMN) leukocytes can be obtained from horse blood by a procedure similar to that previously described for the separation of these leukocytes from human blood. This involved centrifugation of horse blood on a Ficoll-Hypaque medium with a density of 1.095 g/ml. The procedure required approximately 1 h for completion and resulted in the simultaneous preparation of MN (> 98% purity) and PMN (> 96% purity) leukocytes. Cell viability exceeded 95% and cells retained immunological functions.