The molecular epidemiology and evolution of Epstein-Barr virus: sequence variation and genetic recombination in the latent membrane protein-1 gene.
The molecular epidemiology and evolution of Epstein-Barr virus: sequence variation and genetic recombination in the latent membrane protein-1 gene.
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EB 病毒的分子流行病学和进化：潜伏膜蛋白 1 基因的序列变异和遗传重组。
DOI：
10.1086/314672
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Webster-Cyriaque,J
中科院分区：
文献类型：
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作者：
Walling,DM;Shebib,N;Weaver,SC;Nichols,CM;Flaitz,CM;Webster-Cyriaque,J
EB病毒（Epstein-Barr virus，EBV）基因变异的发生和进化至今仍知之甚少。EB病毒潜伏膜蛋白-1（LMP-1）基因序列特别异质，可用作EB病毒基因型鉴定的工具。因此，通过PCR扩增和克隆检查了直接从EB病毒感染的人体组织中获得的LMP-1序列。EBV基因型被定义为来自22种鉴定的LMP-1序列模式中的“毒株”。LMP-1基因遗传多样性的产生有三种分子机制：点突变、序列缺失或重复和同源重组。发现LMP-1基因进化的速率通过与多个EBV毒株共感染而加速。本研究的结果完善了我们对LMP-1序列变异的理解，并能够准确区分独立的EB病毒感染事件和宿主内EB病毒进化的后果。因此，这种基于LMP-1序列的EBV分子流行病学方法将促进宿主内EBV感染、合并感染和持续性的研究。
The phylogeny and evolution of Epstein-Barr virus (EBV) genetic variation are poorly understood. EBV latent membrane protein-1 (LMP-1) gene sequences are especially heterogeneous and may be useful as a tool for EBV genotype identification. Therefore, LMP-1 sequences obtained directly from EBV-infected human tissues were examined by PCR amplification and cloning. EBV genotypes were defined as “strains” from among 22 identified LMP-1 sequence patterns. Three molecular mechanisms were identified by which genetic diversity arises in the LMP-1 gene: point mutation, sequence deletion or duplication, and homologous recombination. The rate of LMP-1 gene evolution was found to be accelerated by coinfection with multiple EBV strains. The results of this study refine our understanding of LMP-1 sequence variation and enable accurate discrimination between independent EBV infection events and the consequence of intrahost EBV evolution. Thus, this LMP-1 sequence-based approach to EBV molecular epidemiology will facilitate the study of intrahost EBV infection, coinfection, and persistence.