Evaluation of a divergent set of SSR markers to predict F1 grain yield performance and grain yield heterosis in maize.
Evaluation of a divergent set of SSR markers to predict F1 grain yield performance and grain yield heterosis in maize.
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评估一组不同的 SSR 标记以预测 F1 玉米产量表现和玉米产量杂种优势。
作者：
O. Smith;H. Sullivan;B. Hobart;S. Wall
先前的工作表明，Stiff Stalk Synthetic 和 Lancaster Sure Crop 杂种优势群体之间的许多多态性位点具有 0.5 或更大的等位基因频率差异。这些基因座也位于在广泛使用的遗传分化自交系 B73 和 Mo17 之间的杂交中检测到产量杂种优势的大型数量性状基因座 (QTL) 的区域。 B73 和 Mo17 代表美国玉米带广泛使用的杂种优势玉米群体之一。据推测，也许那些负责区分杂种优势群体的标记与部分负责杂种优势的基因座相关。本研究的目的是通过来自广泛使用的美国玉米带杂种优势群体的无关亲本的玉米杂交种检验这一假设。在本研究中，检查了两个杂种优势群体之间存在多态性且等位基因频率差异为 0.40 或更大的位点。 F1 谷物产量和谷物产量杂种优势是在一组重复实验中生长的无关亲本之间进行的一组 59 次杂交中确定的。这两个杂种优势群体中具有等位基因频率差异的多态性位点几乎位于先前鉴定为等位基因频率差异在Stiff Stalk Synthetic和Lancaster Sure Crop品系之间为0.5或更大的多态性区域的几乎所有区域。Stiff Stalk Synthetic杂种优势群体在两项研究中是共有的，但Lancaster组则不然。然而，本研究中选定的基因座并没有比使用所有研究基因座所解释的解释更多地解释 F1 谷物产量或谷物产量杂种优势的变异。
Previous work showed that many of the loci polymorphic between the Stiff Stalk Synthetic and Lancaster Sure Crop heterotic group had allele frequency differences of 0.5 or more. These loci were also located in regions where large quantitative trait loci (QTL) have been detected for yield heterosis in the cross between the widely used genetically divergent inbred lines B73 and Mo17. B73 and Mo17 represent one of the widely used heterotic maize groups in the U.S Corn Belt. It was hypothesized that perhaps those markers responsible for distinguishing heterotic groups are linked to loci that are responsible, in part, for heterosis.' The objectives of this study were to test this hypothesis for maize hybrids with unrelated parents from widely used U.S. Corn Belt heterotic groups. In this study loci that were polymorphic between the two heterotic groups and had allele frequency differences of 0.40 or more were examined. F1 grain yield and grain yield heterosis were determined in a set of 59 crosses between unrelated parents that had been grown in a set of replicated experiments. The loci polymorphic with allele frequency differences in these two heterotic groups were located in almost all of the regions identified previously as being polymorphic with allele frequency differences of 0.5 or greater between the Stiff Stalk Synthetic and Lancaster Sure Crop lines, The Stiff Stalk Synthetic heterotic group was common to both studies but the Lancaster group was not. However, the selected loci in this study did not explain significantly more of the variation for F1 grain yield or grain yield heterosis than that explained using all of the loci studied.