Living Electrospun Short Fibrous Sponge via Engineered Nanofat for Wound Healing
Living Electrospun Short Fibrous Sponge via Engineered Nanofat for Wound Healing
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DOI：
10.1007/s42765-022-00229-5
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发表时间：
2022-11
影响因子：
16.1
通讯作者：
Xiaoyun Fu;Juan Wang;D. Qian;Zhaowen Chen;Liang Chen;W. Cui;Yan Wang
中科院分区：
文献类型：
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作者：
Xiaoyun Fu;Juan Wang;D. Qian;Zhaowen Chen;Liang Chen;W. Cui;Yan Wang
活细胞和活性因子是组织修复的两大核心要素，直接影响受损组织的愈合效率。纳米脂肪（NF）可以释放活细胞，如脂肪源性干细胞（ADSC），以及活性生长因子，以促进血管生成，从而实现基于细胞的伤口愈合。本文通过工程化NF对三维（3D）仿生短纤维进行修饰，构建了一种新型活体电纺短纤维海绵。聚多巴胺（PDA）改性的均匀分布赋予了活性海绵稳定的机械性能、可逆的吸水性和优异的粘附性，即使在外力反复压缩和长期水浸泡后也是如此。同时，具有均匀NF修饰的活性静电纺短纤维海绵含有ADSCs等活细胞和血管内皮生长因子（VEGF）等活性生长因子，可有效促进人脐静脉内皮细胞（HUVECs）的管形成。在体内，活性海绵能有效、持续地作用于伤口，充当仿生活性皮肤，防止内部营养物质流失，为组织再生创造舒适有利的微环境，促进糖尿病伤口愈合。因此，通过工程化NF的活电纺短纤维海绵有望在损伤组织中原位活细胞和活性因子实现连续伤口愈合。
Living cells and active factors are the two core elements of tissue repair, directly affecting the healing efficiency of damaged tissue. Nanofat (NF) can release living cells, such as adipose-derived stem cells (ADSCs), as well as active growth factors to promote angiogenesis, thus realizing cell-based wound healing. Herein, a novel living electrospun short fibrous sponge is constructed by modifying three-dimensional (3D) bionic short fibers with engineered NF. The uniform distribution of the polydopamine (PDA) modification endows the living sponges with stable mechanical properties, reversible water absorption and excellent adhesion even after repeated compression by an external force and long-term aqueous immersion. Meanwhile, the living electrospun short fibrous sponges with uniform NF modification contain living cells such as ADSCs and active growth factors such as vascular endothelial growth factor (VEGF), which can effectively promote the tube formation of human umbilical vein endothelial cells (HUVECs). In vivo,the living sponges can effectively and continuously act on wounds and act as a bionic living skin to prevent the loss of internal nutrients, creating a comfortable and favorable microenvironment for tissue regeneration and promoting the healing of diabetic wounds. Therefore, living electrospun short fibrous sponges via engineered NF are expected to achieve continuous wound healing with in situ living cells and active factors in injured tissues.