Relationship between Fc gamma 2b receptor and adenylate cyclase of a murine macrophagelike cell line, P388D1.
Relationship between Fc gamma 2b receptor and adenylate cyclase of a murine macrophagelike cell line, P388D1.
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Fc gamma 2b 受体与鼠巨噬细胞样细胞系 P388D1 腺苷酸环化酶之间的关系。
DOI：
10.1021/bi00388a002
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Suzuki,T
中科院分区：
文献类型：
--
作者：
Hirata,Y;Fernandez-Botran,R;Suzuki,T
Yasuhiko Hirata, Rafael Fernandez-Botran, and Tsuneo Suzuki** Department of Microbiology, University of Kansas Medical Center, Kansas City, Kansas 66103 Received September 4, 1986; Revised Manuscript Received December 10, 1986 abstract: The relationship between Fc receptor specific for IgG2b (Fc72bR) and membrane adenylate cyclase was investigated. The specific binding of IgG2b immune complexes to P388Dt cell surface Fc72bR was found to inhibit the basal, forskolin-stimulated, and NaF-stimulated activities of membrane adenylatecyclase by 53%, 57%, and 31%, respectively. On the other hand, the binding of IgG2a immune complexes to cell surface Fc72aR increased the basal activity about 2.5-fold and the forskolin-and NaF-stimulated activities slightly.The fusion of liposomes containing Fc72bR, which was obtained as phosphatidylcholine (PC) binding protein as previously described, with the eye™ membrane preparations resulted in the marked suppression of membrane adenylate cyclase, whereas the fusion of liposomes containing Fc72a, which was obtained as IgG-binding protein, led to about a 2.7-fold increase. The Fc72bR-mediated inhibition of adenylate cyclase may be due to the temporary change of the lipid environment caused by the action of phospholipase A2, which was previously shown to be associated with Fc72bR, since (1) addition of snake venom phospholipase A2 or cholate-solubilized PC-binding protein to P388D [membrane was found to inhibit adenylate cyclase in a dose-dependent manner,(2) prior treatment of snake venom phospholipase A2 or PC-binding protein with a specific inhibitor,/t-bromophenacyl bromide, significantly reduced theirinhibitory action, and (3) a product of phospholipase A2 action, arachidonic acid, was found to be an effective inhibitor of membrane adenylate
