STROMAL CELL-POPULATIONS IN NECROPSY BONE-MARROW SECTIONS FROM ALLOGENEIC MARROW RECIPIENTS AND NONTRANSPLANT PATIENTS
STROMAL CELL-POPULATIONS IN NECROPSY BONE-MARROW SECTIONS FROM ALLOGENEIC MARROW RECIPIENTS AND NONTRANSPLANT PATIENTS
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DOI：
10.1136/jcp.46.7.611
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发表时间：
1993-07-01
影响因子：
3.4
通讯作者：
SLOANE, JP
中科院分区：
文献类型：
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作者：
DILLY, SA;JAGGER, CJ;SLOANE, JP
目的：比较骨髓移植（BMT）受者与正常人的碱性磷酸酶阳性网状细胞（AL-RC）和巨噬细胞的数量，并探讨其与临床特征的相关性。方法：对18例BMT受者和9例突然死亡的正常人进行尸检，取股骨骨髓切片。通过内源性碱性磷酸酶活性观察AL-RC。采用免疫细胞化学技术，利用抗体EBM/11 （CD68）和内源性酸性磷酸酶活性对巨噬细胞进行染色。计数染色细胞的数量，并表示为总有核细胞的百分比。结果：在两组骨髓组织中，CD68染色的巨噬细胞多于酸性磷酸酶染色的巨噬细胞，表明巨噬细胞的异质性。CD68阳性巨噬细胞百分比值在移植受者中较高（p < 0.01）。至少部分原因是由于造血细胞数量的减少。酸性磷酸酶和碱性磷酸酶阳性细胞的百分比值在两组之间没有差异。为了排除骨髓细胞结构变化的影响，我们比较了基质细胞比例。BMT受者的AL-RC: CD68和酸性磷酸酶：CD68比值均较低，说明BMT后AL-RC和酸性磷酸酶细胞的绝对数量或减少，或CD68细胞增加，或两者兼有。每种细胞类型的数量与移植时给予的细胞剂量、移植后的时间、移植物抗宿主病或感染的存在、骨髓红髓比或外周血白细胞计数没有相关性。完整骨髓中AL-RC与巨噬细胞的比值为0.43，明显高于培养骨髓。结论- al - rc和酸性磷酸酶阳性细胞可能是支持造血的重要细胞，BMT后它们的减少可能导致造血功能的抑制。CD68阳性细胞包括具有多种功能的巨噬细胞，这些细胞可能在骨髓损伤时增加。
Aims-To compare the numbers of alkaline phosphatase positive reticulum cells (AL-RC) and macrophages in bone marrow transplant (BMT) recipients with numbers in normal subjects and to look for correlations with clinical features.Methods-Sections of femoral marrow were obtained at necropsy from 18 BMT recipients and nine normal subjects who had died suddenly. AL-RC were visualised through their endogenous alkaline phosphatase activity. Macrophages were stained by an immunocytochemical technique using the antibody EBM/11 (CD68) and through their endogenous acid phosphatase activity. The numbers of stained cells were counted and expressed as a percentage of total nucleated cells.Results-In both sets of marrow tissue, more macrophages stained for CD68 than for acid phosphatase, indicating macrophage heterogeneity. The percentage value for CD68 positive macrophages was higher among the transplant recipients (p < 0.01). At least in part this was caused by a reduction in haemopoietic cell numbers. Percentage values for acid phosphatase and alkaline phosphatase positive cells did not differ between the two groups. To exclude the effect of changes in marrow cellularity, stromal cell ratios were compared. The AL-RC: CD68 and acid phosphatase:CD68 ratios were both lower in BMT recipients, indicating that after BMT either the absolute number of AL-RC and acid phosphatase cells decreases, or CD68 cells increase, or there is a combination of the two. There was no correlation between the number of each cell type and cell dose given at transplantation, time after transplantation, presence of graft versus host disease or infection, marrow erythroid: myeloid ratio, or peripheral white cell count. The ratio of AL-RC to macrophages in our intact marrow was 0.43, considerably higher than that reported in cultured marrow.Conclusions-AL-RC and acid phosphatase positive cells may be most important for supporting haemopoiesis and their reduction after BMT may contribute to depression of haemopoiesis. CD68 positive cells include macrophages with a wide variety of functions and these may be increased in response to marrow damage.