Nitric Oxide Mitigates Salt Stress by Regulating Levels of Osmolytes and Antioxidant Enzymes in Chickpea.
Nitric Oxide Mitigates Salt Stress by Regulating Levels of Osmolytes and Antioxidant Enzymes in Chickpea.
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DOI：
10.3389/fpls.2016.00347
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发表时间：
2016
影响因子：
5.6
通讯作者：
Tran LS
中科院分区：
文献类型：
--
作者：
Ahmad P;Abdel Latef AA;Hashem A;Abd Allah EF;Gucel S;Tran LS
以供体S-亚硝基-N-乙酰青霉胺(SNAP)为供体，外施一氧化氮(NO)能否减轻盐胁迫对鹰嘴豆的伤害。植物。将Snap(50μM)用于生长在非盐分和盐分(50和100 mMNacl)条件下的鹰嘴豆植株。盐胁迫抑制了鹰嘴豆植株的生长和生物量、叶片相对含水量和叶绿素含量。高盐胁迫增加了鹰嘴豆植株的电解质渗漏、类胡萝卜素含量和渗透调节物质(脯氨酸、甘氨酸甜菜碱、可溶性蛋白质和可溶性糖)、过氧化氢(H_2O_2)和丙二醛(MDA)水平，以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GSH-R)等抗氧化酶活性。在盐胁迫下，鹰嘴豆植株中具有代表性的SOD、CAT和APX基因的表达也被上调。另一方面，与仅用盐处理相比，外源NO处理提高了盐渍植物的生长参数、LRWC、光合色素产量和渗透调节物质水平，以及与所检测的SOD、CAT和APX基因上调相关的抗氧化酶活性。此外，添加NO的盐胁迫植株的电解质渗漏率、H_2O_2和丙二醛含量比单独盐处理的植株有所下降。因此，外源NO保护鹰嘴豆植株免受盐胁迫的伤害，是通过促进抗氧化酶的生物合成，从而促进盐胁迫下的植物生长。综上所述，我们的结果表明，NO能够通过改善LRWC、光合色素生物合成、渗透调节物质积累和抗氧化防御系统来缓解高盐胁迫对鹰嘴豆的不利影响。
This work was designed to evaluate whether external application of nitric oxide (NO) in the form of its donor S-nitroso-N-acetylpenicillamine (SNAP) could mitigate the deleterious effects of NaCl stress on chickpea (Cicer arietinum L.) plants. SNAP (50 μM) was applied to chickpea plants grown under non-saline and saline conditions (50 and 100 mM NaCl). Salt stress inhibited growth and biomass yield, leaf relative water content (LRWC) and chlorophyll content of chickpea plants. High salinity increased electrolyte leakage, carotenoid content and the levels of osmolytes (proline, glycine betaine, soluble proteins and soluble sugars), hydrogen peroxide (H2O2) and malondialdehyde (MDA), as well as the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase in chickpea plants. Expression of the representative SOD, CAT and APX genes examined was also up-regulated in chickpea plants by salt stress. On the other hand, exogenous application of NO to salinized plants enhanced the growth parameters, LRWC, photosynthetic pigment production and levels of osmolytes, as well as the activities of examined antioxidant enzymes which is correlated with up-regulation of the examined SOD, CAT and APX genes, in comparison with plants treated with NaCl only. Furthermore, electrolyte leakage, H2O2 and MDA contents showed decline in salt-stressed plants supplemented with NO as compared with those in NaCl-treated plants alone. Thus, the exogenous application of NO protected chickpea plants against salt stress-induced oxidative damage by enhancing the biosyntheses of antioxidant enzymes, thereby improving plant growth under saline stress. Taken together, our results demonstrate that NO has capability to mitigate the adverse effects of high salinity on chickpea plants by improving LRWC, photosynthetic pigment biosyntheses, osmolyte accumulation and antioxidative defense system.