A Single-Cell Transcriptome Atlas of the Human Pancreas.
A Single-Cell Transcriptome Atlas of the Human Pancreas.
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DOI：
10.1016/j.cels.2016.09.002
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发表时间：
2016-10-26
期刊：
影响因子：
9.3
通讯作者：
van Oudenaarden A
中科院分区：
文献类型：
--
作者：
Muraro MJ;Dharmadhikari G;Grün D;Groen N;Dielen T;Jansen E;van Gurp L;Engelse MA;Carlotti F;de Koning EJ;van Oudenaarden A
为了了解器官的功能，了解它的细胞类型及其相应的标记基因是很重要的。这对于像胰腺这样的人类组织来说是一项特别具有挑战性的任务，因为可靠的标记物是有限的。因此，转录组范围的研究通常是在朗格汉斯的共用胰岛上进行的，掩盖了稀有细胞类型和潜在亚群的贡献。为了克服这一挑战，我们开发了一个自动化平台，使用FACS、机器人技术和CEL-seq2协议从已故器官捐赠者那里获得数千个单个胰腺细胞的转录，从而允许对所有主要类型的胰腺细胞进行电子纯化。我们鉴定了特定细胞类型的转录因子和一组REG3A阳性的腺泡细胞。我们还发现CD24和TM4SF4的表达可用于分选高纯度的活体α细胞和β细胞。这一资源将有助于加深对糖尿病的胰腺生物学和病理生理学的了解。人类胰腺的单细胞测序使细胞类型的电子纯化成为可能。我们提供细胞类型特异的基因、转录因子和细胞表面标记StemID发现腺泡细胞和β细胞的异常群体CD24和TM4SF4功能作为两个标记来丰富α和β细胞。单细胞mRNA测序用于描述成人胰腺细胞类型的转录组。开采这一资源是为了寻找现有细胞类型和标记的亚群，可用于纯化活的阿尔法和贝塔细胞。
To understand organ function, it is important to have an inventory of its cell types and of their corresponding marker genes. This is a particularly challenging task for human tissues like the pancreas, because reliable markers are limited. Hence, transcriptome-wide studies are typically done on pooled islets of Langerhans, obscuring contributions from rare cell types and of potential subpopulations. To overcome this challenge, we developed an automated platform that uses FACS, robotics, and the CEL-Seq2 protocol to obtain the transcriptomes of thousands of single pancreatic cells from deceased organ donors, allowing in silico purification of all main pancreatic cell types. We identify cell type-specific transcription factors and a subpopulation of REG3A-positive acinar cells. We also show that CD24 and TM4SF4 expression can be used to sort live alpha and beta cells with high purity. This resource will be useful for developing a deeper understanding of pancreatic biology and pathophysiology of diabetes mellitus. Single-cell sequencing of human pancreas allows in silico purification of cell types We provide cell-type-specific genes, transcription factors, and cell-surface markers StemID finds outlier populations of acinar and beta cells CD24 and TM4SF4 function as two markers to enrich for alpha and beta cells Single-cell mRNA sequencing was used to describe the transcriptome of adult human pancreatic cell types. This resource was mined to find subpopulations of existing cell types and markers that can be used to purify live alpha and beta cells.
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DOI：
10.1126/science.1247651
发表时间：
2014-02-14
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Jaitin DA;Kenigsberg E;Keren-Shaul H;Elefant N;Paul F;Zaretsky I;Mildner A;Cohen N;Jung S;Tanay A;Amit I
通讯作者：
Amit I
影响因子：
4.6
作者：
Anderson, Keith R.;Singer, Ruth A.;Sussel, Lori
通讯作者：
Sussel, Lori
影响因子：
8.1
作者：
DiGruccio MR;Mawla AM;Donaldson CJ;Noguchi GM;Vaughan J;Cowing-Zitron C;van der Meulen T;Huising MO
通讯作者：
Huising MO
影响因子：
7.7
作者：
Kimple ME;Keller MP;Rabaglia MR;Pasker RL;Neuman JC;Truchan NA;Brar HK;Attie AD
通讯作者：
Attie AD
影响因子：
30.8
作者：
Grant, SFA;Thorleifsson, G;Stefansson, K
通讯作者：
Stefansson, K