Label-free analysis of adsorbed protein heterogeneity on individual particles, based on single particle collision events
Label-free analysis of adsorbed protein heterogeneity on individual particles, based on single particle collision events
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基于单粒子碰撞事件对单个粒子上吸附的蛋白质异质性进行无标记分析
DOI：
10.1016/j.elecom.2020.106666
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发表时间：
2020
影响因子：
5.4
通讯作者：
Mao Lanqun
中科院分区：
文献类型：
--
作者：
Yi Wei;Xu Cong;Xiong Tianyi;Gao Tienan;Yu Ping;He Xiaohua;Mao Lanqun
蛋白质在颗粒表面吸附的异质性研究因其在基础研究和质量控制中的重要作用而引起了人们极大的研究兴趣。在这里，我们首次报道了一种新的无标记分析蛋白质在单个纳米颗粒上吸附的异质性的方法，该方法基于纳米管孔口处的颗粒碰撞事件。停留时间强烈依赖于蛋白质在聚苯乙烯颗粒上的吸附数量，这可以用来分析蛋白质在单颗粒水平上的吸附的不均一性。该方法具有无标记、灵敏、重复性好、操作简单等特点，为研究纳米颗粒表面物理化学参数的非均质性开辟了一条新的途径。
Investigation of the heterogeneity of protein adsorption on particle surfaces has attracted enormous research attention owing to its great importance in fundamental studies and quality control. Herein we, for the first time, report a new method for label-free analysis of the heterogeneity of protein adsorption on single nanoparticles, based on particle collision events at the orifice of a nanopipette. The dwell time was strongly dependent on the amount of protein adsorbed on polystyrene particles, which could be used to analyze the heterogeneity of protein adsorption at the single particle level. This method presents a label-free, sensitive, reproducible and easily-operated way to analyze adsorption behavior at the single particle level, which opens a new approach to the study of the heterogeneity of physicochemical parameters at the surface of nanoparticles.