ALTERATION OF THE RNA-POLYMERASE SPECIFICITY OF U3 SNRNA GENES DURING EVOLUTION AND INVITRO
ALTERATION OF THE RNA-POLYMERASE SPECIFICITY OF U3 SNRNA GENES DURING EVOLUTION AND INVITRO
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DOI：
10.1016/0092-8674(91)90469-f
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发表时间：
1991-05-03
期刊：
影响因子：
64.5
通讯作者：
FILIPOWICZ, W
中科院分区：
文献类型：
--
作者：
KISS, T;MARSHALLSAY, C;FILIPOWICZ, W
我们目前的证据表明，在植物中编码U3 snRNA的基因是由RNA聚合酶III（polIII），而不是由RNA聚合酶II（polII）在脊椎动物或低等真核生物转录。 U3基因是唯一已知的在不同生物体中由不同聚合酶转录的基因的例子。 通过操纵启动子元件之间的间隔并插入pol II特异性终止信号，可以将植物U3基因转化为功能性pol II转录基因。 Pol II转录的U3 RNA，含有与野生型对应物中存在的不同的5 '端帽，在转染的原生质体中包装成可用抗原纤蛋白抗体沉淀的U3 snRNP。 这些发现进一步证明了pol II和pol III转录系统的共同祖先，并表明某些基因的启动子多样化是最近才发生的。
We present evidence that the genes encoding U3 snRNA in plants are transcribed by RNA polymerase III (pol III) and not by RNA polymerase II (pol II) as in vertebrates or lower eukaryotes. The U3 gene is the only known example of a gene transcribed by different polymerases in different organisms. It is possible to convert the plant U3 gene into a functional pol II-transcribed gene by manipulating the spacing between the promoter elements and inserting a pol II-specific termination signal. Pol II-transcribed U3 RNA, containing the 5'-terminal cap different from that present in the wild-type counterpart, is packaged in transfected protoplasts into U3 snRNP precipitable with anti-fibrillarin antibodies. These findings provide further evidence for the common ancestry of the pol II and pol III transcription systems, and indicate that promoter diversification in some genes has occurred relatively recently.