The linkage disequilibrium between chloroplast DNA and mitochondrial DNA haplotypes in Beta vulgaris ssp maritima (L.):: the usefulness of both genomes for population genetic studies
The linkage disequilibrium between chloroplast DNA and mitochondrial DNA haplotypes in Beta vulgaris ssp maritima (L.):: the usefulness of both genomes for population genetic studies
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DOI：
10.1046/j.1365-294x.2000.00843.x
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发表时间：
2000-02-01
影响因子：
4.9
通讯作者：
van Dijk, H
中科院分区：
文献类型：
--
作者：
Desplanque, B;Viard, F;van Dijk, H
植物线粒体基因组的结构和进化可能允许相同的线粒体变异在不同种群中反复出现。在使用这些标记进行群体遗传分析时，相同的线粒体变异是通过迁移分布还是通过突变（产生同质性）在不同群体中反复出现是一个重要的问题。叶绿体和线粒体（即两个母系遗传的细胞质基因组）之间的遗传关联可能表明线粒体基因组是否发生同质化。对来自法国和西班牙的野生甜菜种群的414个个体进行了线粒体和叶绿体多态性筛选。采用限制性内切片段长度多态性（RFLP）分析线粒体DNA （mtDNA）多态性，采用聚合酶链反应PCR-RFLP分析叶绿体DNA （cpDNA）多态性，采用通用引物扩增。mtDNA和cpDNA分别有20个和13个变异。大多数表现出广泛的地理分布。由于估计mtDNA和cpDNA单倍型之间存在非常强的连锁不平衡，因此排除了甜菜线粒体基因组的高复发突变率。在不同地区的人群中发现的相同的线粒体变异可能是移民的结果。本研究认为，当需要母系标记在大区域范围内进行甜菜群体遗传学分析时，mtDNA是与cpDNA一样有价值的工具。
The structure and evolution of the plant mitochondrial genome may allow recurrent appearance of the same mitochondrial variants in different populations. Whether the same mitochondrial variant is distributed by migration or appears recurrently by mutation (creating homoplasy) in different populations is an important question with regard to the use of these markers for population genetic analyses. The genetic association observed between chloroplasts and mitochondria (i.e. two maternally inherited cytoplasmic genomes) may indicate whether or not homoplasy occurs in the mitochondrial genome. Four-hundred and fourteen individuals sampled in wild populations of beets from France and Spain were screened for their mitochondrial and chloroplast polymorphisms. Mitochondrial DNA (mtDNA) polymorphism was investigated with restriction fragment length polymorphism (RFLP) and chloroplast DNA (cpDNA) polymorphism was investigated with polymerase chain reaction PCR-RFLP, using universal primers for the amplification. Twenty and 13 variants for mtDNA and cpDNA were observed, respectively. Most exhibited a widespread geographical distribution. As a very strong linkage disequilibrium was estimated between mtDNA and cpDNA haplotypes, a high rate of recurrent mutation was excluded for the mitochondrial genome of beets. Identical mitochondrial variants found in populations of different regions probably occurred as a result of migration. We concluded from this study that mtDNA is a tool as valuable as cpDNA when a maternal marker is needed for population genetics analyses in beet on a large regional scale.