Selective replenishment of two vesicle pools depends on the source of Ca2+ at the Drosophila synapse
Selective replenishment of two vesicle pools depends on the source of Ca2+ at the Drosophila synapse
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DOI：
10.1016/s0896-6273(02)00777-8
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发表时间：
2002-07-18
期刊：
影响因子：
16.2
通讯作者：
Kidokoro, Y
中科院分区：
文献类型：
--
作者：
Kuromi, H;Kidokoro, Y
在突触囊泡（SV）经历胞吐作用之后，SV池通过在神经末梢处回收SV来补充。在果蝇神经肌肉突触中，有两个不同的SV池（即，主要维持突触传递的外/内循环池（ECP），和仅在强直刺激期间参与突触传递的储备池（RP））。标记内吞囊泡结构与荧光苯乙烯基染料，FM 1 -43，并测量细胞内呼叫浓度与Ca 2+指示剂，rhod-2，我们在这里表明，ECP补充SV内吞刺激过程中，这个过程取决于外部的Ca 2+。相反，RP是重新填充破伤风停止后的过程介导的Ca 2+从内部存储释放。
After synaptic vesicles (SVs) undergo exocytosis, SV pools are replenished by recycling SVs at nerve terminals. At Drosophila neuromuscular synapses, there are two distinct SV pools (i.e., the exo/endo cycling pool (ECP), which primarily maintains synaptic transmission, and the reserve pool (RP), which participates in synaptic transmission only during tetanic stimulation). Labeling endocytosed vesicular structures with a fluorescent styryl dye, FM1-43, and measuring intracellular Call concentrations with a Ca2+ indicator, rhod-2, we show here that the ECP is replenished by SVs endocytosed during stimulation, and this process depends on external Ca2+. In contrast, the RP is refilled after cessation of tetanus by a process mediated by Ca2+ released from internal stores.