ABA-Dependent and -Independent G-Protein Signaling in Arabidopsis Roots Revealed through an iTRAQ Proteomics Approach
ABA-Dependent and -Independent G-Protein Signaling in Arabidopsis Roots Revealed through an iTRAQ Proteomics Approach
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DOI：
10.1021/pr2001786
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发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Pandey, Sona
中科院分区：
文献类型：
--
作者：
Alvarez, Sophie;Hicks, Leslie M.;Pandey, Sona
异源三聚体G蛋白是真核生物中重要的信号转导蛋白。植物激素脱落酸（阿坝）已成为植物中G蛋白介导的信号转导途径的关键调节剂。G蛋白信号的ABA调节涉及传统和新的机制。我们利用拟南芥G蛋白α亚基gpa1的无效突变体来评估在何种程度上ABA依赖的变化在蛋白质组中的G蛋白的调节。我们使用拟南芥根组织作为阿坝和G蛋白，单独和组合，影响根的生长和发育。我们鉴定了720种蛋白质，其中42种表现出GPA1依赖性，74种表现出ABA依赖性丰度变化。大多数ABA调节的蛋白质也是GPA1依赖的。我们的数据提供了对ABA调节的G蛋白信号传导如何实现组织特异性的深入了解。在gpa1突变体中，许多与ER体形成和细胞内运输相关的蛋白质发生了改变，表明GPA1在这些通路中具有新的作用。阿坝代谢和阿坝信号之间的潜在联系也被揭示：在阿坝的情况下，蛋白质丰度的变化的比较提供了线索，在ABA独立的信号通路，例如，调节细胞分裂的GPA 1的作用。这些发现大大有助于我们了解植物中G蛋白的信号转导机制。
Heterotrimeric G-proteins are important signal transducers in all eukaryotes. The plant hormone abscisic acid (ABA) has emerged as a key regulator of G-protein-mediated signaling pathways in plants. ABA-regulation of G-protein signaling involves both conventional and novel mechanisms. We have utilized the null mutant of the Arabidopsis G-protein alpha subunit gpa1 to evaluate to what extent ABA-dependent changes in the proteome are regulated by G-proteins. We used Arabidopsis root tissue as both ABA and G-proteins, individually and in combination, affect root growth and development. We identified 720 proteins, of which 42 showed GPA1-dependent and 74 showed ABA-dependent abundance changes. A majority of ABA-regulated proteins were also GPA1-dependent. Our data provide insight into how tissue specificity might be achieved in ABA-regulated G-protein signaling. A number of proteins related to ER body formation and intracellular trafficking were altered in gpa1 mutant, suggesting a novel role for GPA1 in these pathways. A potential link between ABA metabolism and ABA signaling was also revealed: The comparison of protein abundance changes in the absence of ABA offers clues to the role of GPA1 in ABA-independent signaling pathways, for example, regulation of cell division. These findings substantially contribute to our knowledge of G-protein signaling Mechanisms in plants.