GAMETOCYTOGENESIS BY MALARIA PARASITES IN CONTINUOUS CULTURE
GAMETOCYTOGENESIS BY MALARIA PARASITES IN CONTINUOUS CULTURE
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DOI：
10.1038/286490a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
KRISHNA, G
中科院分区：
文献类型：
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作者：
KAUSHAL, DC;CARTER, R;KRISHNA, G
疟疾寄生虫的无性增殖是通过红细胞内的寄生虫繁殖来进行的。宿主细胞破裂后，释放的裂殖子重新侵入其他红细胞。当再次入侵时，一部分裂殖子不再成为无性寄生虫，而是成为配子体，即对蚊子媒介具有感染性的有性阶段。无性寄生虫向配子体的转化不仅发生在自然感染期间，而且发生在连续体外培养中，如 Trager 和 Jensen1-4 以及其他人首先报道的 5,6。我们之前表明，恶性疟原虫在培养物中发育成配子体的早期红细胞内阶段（环状阶段）的比例受到培养条件的影响7。在支持快速增殖的条件下，配子体形成很少见，但当寄生虫密度静态时，配子体形成很频繁。我们现在表明，在静态培养物中，近 100% 的环阶段响应 1 mM 环 AMP 发育成配子体，而在快速生长的培养物中，很少环响应环 AMP 发育成配子体。
Asexual proliferation of malaria parasites proceeds by multiplication of the parasites within red cells. Following rupture of the host cells the released merozoites re-invade other red cells. On re-invasion, a proportion of merozoites become, not asexual parasites but gametocytes, the sexual stages infective to the mosquito vectors. Conversion of asexual parasites to gametocytes occurs not only during natural infections but also in continuousin vitroculture as reported first by Trager and Jensen1–4and by others5,6. We showed previously that the proportion of early intra-erythrocytic stages (ring stages) ofPlasmodium falciparumwhich developed into gametocytes in culture was influenced by culture conditions7. Gametocyte formation was rare in conditions supporting rapid proliferation but frequent when parasite densities were static. We now show that nearly 100% of ring stages develop into gametocytes in response to 1 mM cyclic AMP in static cultures whereas in rapidly growing cultures few rings become gametocytes in response to cyclic AMP.