Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast
Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast
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DOI：
10.1111/j.1365-2443.2011.01571.x
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发表时间：
2012-01-01
期刊：
影响因子：
2.1
通讯作者：
Aiba, Hirofumi
中科院分区：
文献类型：
--
作者：
Ohtsuka, Hokuto;Azuma, Kenko;Aiba, Hirofumi
ecl1 +、ecl2+和ecl3+基因编码高度同源的小分子蛋白，它们的过量表达使裂殖酵母既能抵抗H2O2胁迫，又能延长其寿命。然而，这些Ecl1家族蛋白的功能机制尚未阐明。在这项研究中，我们进行了微阵列分析，并确定参与性发育和应激反应的基因的表达受到Ecl1家族蛋白过度表达的影响。与mRNA表达谱一致，过表达Ecl 1家族蛋白的细胞表现出高的交配效率和对H2O2的抗性表型。我们发现，H2O2抗性表型依赖于过氧化氢酶Ctt1，并且Ctt1+的过度表达不影响时间寿命。此外，我们发现，6个基因，ste11+，spk1+，hsr1+，rsv2+，hsp9+和lsd90+，其表达增加的细胞过度表达Ecl1家族蛋白质参与的时间寿命在裂殖酵母。在这些基因中，ste11+和hsr1+的诱导依赖于转录因子Prr1，我们发现，在Prr1缺失突变体中，Ecl1家族蛋白质对时间寿命的延长显著减少。从这些结果，我们建议，Ecl1家族蛋白进行H2O2胁迫抗性和时间寿命延长ctt1+和prr1+依赖的方式，分别。
ecl1 +, ecl2+ and ecl3+ genes encode highly homologous small proteins, and their over-expressions confer both H2O2 stress resistance and chronological lifespan extension on Schizosaccharomyces pombe. However, the mechanisms of how these Ecl1 family proteins function have not been elucidated. In this study, we conducted microarray analysis and identified that the expression of genes involved in sexual development and stress responses was affected by the over-expression of Ecl1 family proteins. In agreement with the mRNA expression profile, the cells over-expressing Ecl1 family proteins showed high mating efficiency and resistant phenotype to H2O2. We showed that the H2O2-resistant phenotype depends on catalase Ctt1, and over-expression of ctt1+ does not affect chronological lifespan. Furthermore, we showed that six genes, ste11+, spk1+, hsr1+, rsv2+, hsp9+ and lsd90+, whose expressions are increased in cells over-expressing Ecl1 family proteins are involved in chronological lifespan in fission yeast. Among these genes, the induction of ste11+ and hsr1+ was dependent on a transcription factor Prr1, and we showed that the extensions of chronological lifespan by Ecl1 family proteins are remarkably diminished in prr1 deletion mutant. From these results, we propose that Ecl1-family proteins conduct H2O2 stress resistance and chronological lifespan extension in ctt1+- and prr1+-dependent manner, respectively.