Cellular Interactions and Fatty Acid Transporter CD36-Mediated Uptake of Per- and Polyfluorinated Alkyl Substances (PFAS)
Cellular Interactions and Fatty Acid Transporter CD36-Mediated Uptake of Per- and Polyfluorinated Alkyl Substances (PFAS)
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DOI：
10.1021/acs.chemrestox.2c00078
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发表时间：
2022-04-18
影响因子：
4.1
通讯作者：
Atilla-Gokcumen, G. Ekin
中科院分区：
文献类型：
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作者：
Camdzic, Michelle;Aga, Diana S.;Atilla-Gokcumen, G. Ekin
全氟化和多氟化烷基物质（PFAS）是一类广泛用于商业和工业应用的化合物。由于其广泛的用途和化学稳定性，PFAS在环境中持久存在，并在人类和野生动物中生物累积。PFAS暴露与几种负面健康影响有关，包括各种癌症的形成，内分泌系统的破坏和肥胖。然而，在理解PFAS的结构差异如何影响它们在生物系统内的相互作用方面存在重大差距。在这项研究中，我们研究了PFAS在人视网膜上皮细胞中的毒性，其链长、头基和降解程度不同。我们专注于氟调聚物和完全氟化的磺酸盐和羧酸盐，并测量其吸收。我们的研究结果表明，磺酸盐采取了更高的水平相比，其氟调聚物和羧酸盐的对应物。此外，具有8和10个碳（C8和C10）的PFAS与具有6个碳（C6）的PFAS相比以更高的水平被吸收。我们还研究了脂肪酸转运蛋白CD36在PFAS摄取中的作用，发现CD36水平升高导致细胞中PFAS水平升高。总的来说，我们的结果表明PFAS的头基结构影响毒性，磺酸盐比羧酸盐诱导更高的细胞活力降低（类似于50%）。我们的研究结果还将CD36的活性与PFAS摄取到细胞中联系起来。
Per- and polyfluorinated alkyl substances (PFAS) are a class of widely used compounds in an array of commercial and industrial applications. Due to their extensive use and chemical stability, PFAS persist in the environment and bioaccumulate in humans and wildlife. PFAS exposure have been linked to several negative health effects, including the formation of various cancers, disruption of the endocrine system, and obesity. However, there is a major gap in understanding how structural differences in PFAS impact their interactions within a biological system. In this study, we examined the toxicity of PFAS with differences in chain length, head group, and degree of fluorination in human retinal epithelial cells. We focused on fluorotelomeric and fully fluorinated sulfonates and carboxylates and measured their uptake. Our results showed that sulfonates are taken up at higher levels as compared to their fluorotelomer and carboxylate counterparts. Furthermore, PFAS with 8 and 10 carbons (C8 and C10) are taken up at a higher level compared to those with six carbons (C6). We also investigated the role of the fatty acid transporter CD36 in PFAS uptake and found that increased CD36 levels result in higher levels of PFAS in cells. Overall, our results suggest that the head group structure of PFAS impacts toxicity, with sulfonates inducing a higher decrease in cell viability (similar to 50%) than carboxylates. Our results also link the activity of CD36 to PFAS uptake into cells.