A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer)
A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer)
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DOI：
10.1111/j.1365-3024.2006.00862.x
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发表时间：
2006-08-01
影响因子：
2.2
通讯作者：
McDevitt, R.
中科院分区：
文献类型：
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作者：
Nisbet, A. J.;Huntley, J. F.;McDevitt, R.
原肌球蛋白是一种致敏性的肌动蛋白结合蛋白，是几种寄生虫的候选疫苗。用聚合酶链式反应（PCR）从鸡皮虫RNA中扩增得到原肌球蛋白cDNA，编码的预测蛋白与小乳杆菌（Boophilus microplus）和长角血蜱（haemaphyysalis longicornis）原肌球蛋白的同源性分别为89%和88%，与尘螨（HDM）原肌球蛋白derp10的同源性分别为85%。小鼠抗HDM原肌球蛋白抗体在鸡D. gallinae提取物的Western blots上与38 kDa的条带发生反应，与鸡D. gallinae原肌球蛋白的分子质量一致，与鸡D. gallinae原肌球蛋白cDNA编码的推定产物一致。当用同样制备的鸡戎螨蛋白与受感染母鸡的血清进行免疫印迹试验时，血清中的IgY成分与许多螨蛋白结合，但不与原肌球蛋白结合，这表明母鸡在自然感染期间没有直接暴露于这种过敏原。螨虫原肌球蛋白的免疫定位表明，原肌球蛋白在螨虫组织中普遍存在。免疫定位和免疫印迹也表明家禽红螨摄取宿主IgY。
Tropomyosin is an allergenic, actin-binding protein and a proposed vaccine candidate from several species of parasite. Tropomyosin cDNA, obtained by polymerase chain reaction (PCR) amplification from Dermanyssus gallinae RNA, encoded a predicted protein with 89% and 88% identity to tropomyosins from the ticks Boophilus microplus and Haemaphysalis longicornis, respectively, and 85% identity to the house dust mite (HDM) tropomyosin Der p 10. Mouse antibodies raised against HDM tropomyosin reacted with a band of 38 kDa on Western blots of D. gallinae extract, consistent with the molecular masses of acarine tropomyosins and the putative product of the cDNA encoding D. gallinae tropomyosin. When the same preparation of D. gallinae proteins was used in Western blots with serum from infested hens, the IgY component of the serum bound to a number of mite proteins, but not to tropomyosin, indicating that hens are not directly exposed to this allergen during a natural infestation. Immunolocalization of tropomyosin in mites indicated a ubiquitous distribution of the molecule in mite tissues. Immunolocalization and Western blotting also indicated that poultry red mites ingest host IgY.