Epstein-Barr virus DNA load in cerebrospinal fluid and plasma of patients with AIDS-related lymphoma
Epstein-Barr virus DNA load in cerebrospinal fluid and plasma of patients with AIDS-related lymphoma
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DOI：
10.1080/13550280260422730
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发表时间：
2002-10-01
影响因子：
3.2
通讯作者：
Falk, KI
中科院分区：
文献类型：
--
作者：
Bossolasco, S;Cinque, P;Falk, KI
脑脊液中EB病毒(EBV)DNA的检测与获得性免疫缺陷综合征(AIDS)相关的脑淋巴瘤有关。对42例艾滋病相关性非霍奇金淋巴瘤(NHL)患者的脑脊液和血浆中EB病毒DNA进行了实时定量聚合酶链式反应(PCR)检测。原发性中枢神经系统淋巴瘤(PCNSL)20例，全身性NHL 22例，其中中枢神经系统受累(CNS-NHL)12例。并以16例HIV感染合并其他中枢神经系统疾病的患者为对照。20例PCNSL患者中有16例(80%)脑脊液中检出EBV DNA，7例(32%)系统性NHL患者中检出EBV DNA，8例(67%)CNS-NHL患者脑脊液中检出EBV DNA，2例正常人脑脊液中检出EBV DNA。PCNSL或CNS-NHL患者脑脊液中病毒EBV DNA水平显著高于系统性NHL患者和对照组。5/16例PCNSL患者、9/16例系统性NHL患者、4/9例CNS-NHL患者和4/15例正常对照的血浆中EBV DNA阳性。患者组之间的血浆病毒载量没有差异。在CNS-NHL患者中，在CNS受累之前采集的血浆样本中的EBV DNA水平显著高于未继发CNS受累的全身性NHL患者。接受抗疱疹药物治疗的患者脑脊液中的EBV DNA水平显著低于未治疗患者，但血浆中的EBV DNA水平并不显著。在HIV相关的脑淋巴瘤中发现高水平的脑脊液EBV DNA，病毒载量可能在临床上有用。血浆EBVDNA水平升高可能预示中枢神经系统受累于系统性NHL。
Detection of Epstein-Barr virus (EBV) DNA in the cerebrospinal fluid (CSF) is associated with acquired immunodeficiency syndrome (AIDS)-related brain lymphoma. Real-time polymerase chain reaction (PCR) was performed to quantify EBV DNA in CSF and plasma from 42 patients with AIDS-related non-Hodgkin's lymphoma (NHL). Twenty patients had primary central nervous system lymphoma (PCNSL) and 22 systemic NHL, including 12 with central nervous system involvement (CNS-NHL). As controls, 16 HIV-infected patients with other CNS disorders were examined. EBV DNA was detected in the CSF from 16/20 (80%) patients with PCNSL, 7/22 (32%) with systemic NHL, 8/12 (67%) with CNS-NHL, and 2/16 (13%) of the controls. The viral EBV DNA levels were significantly higher in the CSF from patients with PCNSL or CNS-NHL compared to patients with systemic NHL or controls. EBV DNA was detected in plasma from 5/16 (31%) patients with PCNSL, 9/16 (56%) with systemic NHL, 4/9 (44%) with CNS-NHL, and 4/15 (27%) controls. No difference in plasma viral load was found between patient groups. From the patients with CNS-NHL, plasma samples drawn prior to CNS involvement contained significantly higher EBV DNA levels than those from systemic NHL patients without subsequent CNS involvement. EBV DNA levels in the CSF, but not in plasma, from patients treated with antiherpes drugs were significantly lower than in untreated patients. High CSF EBV DNA levels were found in HIV-associated brain lymphomas and the viral load can be clinically useful. High plasma EBV DNA levels might predict CNS involvement in systemic NHL.