Cordycepin and pentostatin biosynthesis gene identified through transcriptome and proteomics analysis of Cordyceps kyushuensis Kob.
Cordycepin and pentostatin biosynthesis gene identified through transcriptome and proteomics analysis of Cordyceps kyushuensis Kob.
复制标题
DOI：
10.1016/j.micres.2018.09.005
复制
发表时间：
2019
影响因子：
6.7
通讯作者：
Xuan Zhao;Guoying Zhang;Caiyi Li;J. Ling
中科院分区：
文献类型：
--
作者：
Xuan Zhao;Guoying Zhang;Caiyi Li;J. Ling
九州虫草是唯一生长在双线虫草幼虫上的虫草品种，已被证明含有虫草素等多种药理成分。虫草素具有多种药理作用，但在体内可被腺苷脱氨酶转化为3'-脱氧肌苷，从而削弱了虫草素的药效。据报道，喷司他丁可抑制腺苷脱氨酶，与虫草素结合可增强虫草素体内的效率。在九州冬虫夏草的转录组和蛋白质组学分析中，使用BLAST鉴定了一个包含四个基因的单一基因簇，我们将其命名为ck1-ck4，可以合成虫草素和喷司他丁。同时，对转录组和蛋白质组进行KEGG、KOG、GO分析和差异表达基因分析。本研究首先对C.的转录组和蛋白质组进行了测序。并证明存在与虫草素和喷司他丁生物合成相关的单一基因簇，可用于提高虫草素的产量并寻找更多的功能蛋白。
Cordyceps kyushuensisis the only species of cordyceps growing on the larvae ofClanis bilineataWalker, and has been demonstrated that there are lots of pharmacological components including cordycepin. Cordycepin shows lots of pharmacological action but it could be converted to 3′-deoxyinosine by adenosine deaminasein vivo, which weakens the efficiency of cordycepin. That pentostatin, which has been reported to inhibit adenosine deaminase, combining cordycepin could enhance the efficiency of cordycepinin vivo. During transcriptome and proteomics analysis ofCordyceps kyushuensis, a single gene cluster including four genes we named ck1-ck4 which can synthesis both cordycepin and pentostatin has been identified using BLAST. Meanwhile, KEGG, KOG, GO analysis and differentially expressed genes were analyzed in transcriptome and proteomics. This study first sequenced transcriptome and proteomics ofC. kyushuensis, and demonstrated that there is a single gene cluster related to biosynthesis of cordycepin and pentostatin, which can be employed to improve the yield of cordycepin and find more functional proteins.