Parallel stimulation in adipocytes of the plasma membrane Ca2+-transport/(Ca2+ + Mg2+)-ATPase system and mitochondrial pyruvate dehydrogenase by a supernatant factor derived from isolated plasma membranes.
Parallel stimulation in adipocytes of the plasma membrane Ca2+-transport/(Ca2+ + Mg2+)-ATPase system and mitochondrial pyruvate dehydrogenase by a supernatant factor derived from isolated plasma membranes.
复制标题
通过源自分离质膜的上清液因子平行刺激脂肪细胞中的质膜Ca2-转运/(Ca2Mg2)-ATP酶系统和线粒体丙酮酸脱氢酶。
DOI：
10.1016/s0006-291x(81)80252-5
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发表时间：
1981
影响因子：
3.1
通讯作者：
Jarett,L
中科院分区：
文献类型：
--
作者：
McDonald,JM;Pershadsingh,HA;Kiechle,FL;Jarett,L
一种来源于脂肪细胞质膜上清液的因子，模拟胰岛素对线粒体丙酮酸脱氢酶的影响(Kiechle，F.L.，Jarett，L.，Kotagal，N.和Popp，D.A.(1981)J.Biol.化学。256,2945-2951)激活高亲和力的(Ca~(2+)+Mg~(2+))-ATPase和依赖于ATP的Ca~(2+)转运。上清液经G25 Sephadex柱层析分离，得到4个失活组分和1个活性组分。所有组分在激活丙酮酸脱氢酶、(Ca~(2+)-Mg~(2+))-ATPase和Ca~(2+)转运方面表现出相同的表现，表明激活线粒体丙酮酸脱氢酶的活性部分也负责刺激质膜上的钙依赖过程。
A factor derived from the supernatant of adipocyte plasma membranes which mimics the effects of insulin on mitochondrial pyruvate dehydrogenase (Kiechle, F.L., Jarett, L., Kotagal, N., and Popp, D.A. (1981) J. Biol. Chem. 256, 2945–2951) was shown to activate a high affinity (Ca2++ Mg2+)-ATPase and ATP-dependent Ca2+transport of adipocyte plasma membranes. Fractionation of the supernatant by G25 Sephadex chromatography separated the material into four inactive and one active fraction. All of the fractions behaved identically in terms of activation of pyruvate dehydrogenase, (Ca2++ Mg2+)- ATPase, and Ca2+transport, suggesting that the active fraction responsible for stimulating mitochondrial pyruvate dehydrogenase was also responsible for stimulating the plasma membrane Ca2+-dependent processes.