Automated Decellularization of Musculoskeletal Tissues with High Extracellular Matrix Retention
Automated Decellularization of Musculoskeletal Tissues with High Extracellular Matrix Retention
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DOI：
10.1089/ten.tec.2022.0005
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发表时间：
2022-04-01
影响因子：
3
通讯作者：
Detamore, Michael S.
中科院分区：
文献类型：
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作者：
Hamilton, Alex G.;Townsend, Jakob M.;Detamore, Michael S.
手动组织脱细胞化是一个繁重的过程，需要操作者应用许多连续的处理，并且可能容易出现用户错误和结果变化。虽然自动化去细胞化设备以前已经报道过，但近年来在开源平台方面取得了进展，以前的自动化去细胞化设备一直依赖于闭源和专有的硬件或软件组件。目前工作的目的是开发和验证一个完整的开源自动脱细胞系统，供其他人采用。开源脱细胞装置是一种低成本（
Manual tissue decellularization is an onerous process that requires the application of many sequential treatments by an operator and can be prone to user error and result variability. While automated decellularization devices have been previously reported, with advances being made in recent years toward open-source platforms, previous automated decellularization devices have been reliant on hardware or software components that are closed-source and proprietary. The aim of the current work was to develop and validate a full open-source automated decellularization system to be available for others to adopt. The open-source decellularization apparatus is a low-cost (