Effect of a diet-induced n-3 PUFA depletion on cholinergic parameters in the rat hippocampus
Effect of a diet-induced n-3 PUFA depletion on cholinergic parameters in the rat hippocampus
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DOI：
10.1194/jlr.m300079-jlr200
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发表时间：
2003-08-01
影响因子：
6.5
通讯作者：
Lavialle, M
中科院分区：
文献类型：
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作者：
Aïd, S;Vancassel, S;Lavialle, M
由于脑细胞膜含有大量的二十二碳六烯酸（DHA，22：6 n-3），（n-3）PUFA饮食缺乏会导致啮齿动物的注意力，学习和记忆能力受损，我们研究了（n-3）PUFA剥夺饮食对中枢胆碱能神经传递系统的影响。我们已经集中在几个胆碱能神经化学参数在额叶皮层和海马的大鼠（n-3）PUFA缺乏的饮食，与大鼠喂养的控制饮食。（n-3）PUFA缺乏导致两个脑区的膜磷脂组成发生变化，DHA急剧损失（62-77%）。然而，胆碱能通路仅在海马中被修改，而不是在额叶皮质中。基础乙酰胆碱（ACh）的释放在海马缺陷大鼠显着（72%）高于对照组，而KCl诱导的释放较低（34%）。（n-3）PUFA剥夺也导致毒蕈碱受体结合减少10%。与此相反，乙酰胆碱酯酶活性和囊泡乙酰胆碱转运蛋白在两个大脑区域是不变的。因此，我们证明了（n-3）PUFA缺乏的饮食可以影响胆碱能神经传递，可能是通过改变磷脂PUFA组成。
Because brain membranes contain large amounts of docosahexaenoic acid (DHA, 22:6n-3), and as (n-3) PUFA dietary deficiency can lead to impaired attention, learning, and memory performance in rodents, we have examined the influence of an (n-3) PUFA-deprived diet on the central cholinergic neurotransmission system. We have focused on several cholinergic neurochemical parameters in the frontal cortex and hippocampus of rats fed an (n-3) PUFA-deficient diet, compared with rats fed a control diet. The (n-3) PUFA deficiency resulted in changes in the membrane phospholipid compositions of both brain regions, with a dramatic loss (62-77%) of DHA. However, the cholinergic pathway was only modified in the hippocampus and not in the frontal cortex. The basal acetylcholine (ACh) release in the hippocampus of deficient rats was significantly (72%) higher than in controls, whereas the KCl-induced release was lower (34%). The (n-3) PUFA deprivation also caused a 10% reduction in muscarinic receptor binding. In contrast, acetylcholinesterase activity and the vesicular ACh transporter in both brain regions were unchanged. jlr Thus, we evidenced that an (n-3) PUFA-deficient diet can affect cholinergic neurotransmission, probably via changes in the phospholipid PUFA composition.