Antioxidant enzyme levels in oral squamous cell carcinoma and normal human oral epithelium.
Antioxidant enzyme levels in oral squamous cell carcinoma and normal human oral epithelium.
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口腔鳞状细胞癌和正常人口腔上皮中的抗氧化酶水平。
DOI：
10.1034/j.1600-0714.2002.310202.x
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Oberley,LW
中科院分区：
文献类型：
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作者：
Yang,J;Lam,EWN;Hammad,HM;Oberley,TD;Oberley,LW
背景：抗氧化酶（含锰和铜锌的超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）限制活性氧诱导的细胞损伤。这项研究的目的是确定人类口腔鳞状细胞癌是否改变了抗氧化酶水平。本研究首次在人口腔粘膜和鳞状细胞癌中进行这项任务。方法：采用半定量免疫组织化学方法检测26例存档的口腔鳞状细胞癌活检标本。检查了14个高分化和12个低分化肿瘤，以及12个口腔粘膜标本。所有切片均由两名口腔颌面病理学家审查，并使用NIH Image对免疫染色切片进行图像分析。抗氧化酶染色强度进行了比较，在不同组的邓肯的多范围test.Results：在一般情况下，粘膜基底细胞显示较低的抗氧化酶水平比棘细胞，和原发性肿瘤细胞显示较低的抗氧化酶染色强度比正常细胞对应。此外，低分化肿瘤细胞显示出比高分化肿瘤细胞更低的抗氧化酶染色强度。含锰超氧化物歧化酶染色强度，但是，高分化的口腔鳞状细胞癌比正常细胞origin.Conclusions：抗氧化酶的检测可能是一个有用的未来标记在口腔癌的分子诊断。此外，它可能不仅是监测化学预防和治疗策略在口腔癌中使用这些酶的有效性，但监测肿瘤复发。
Background:The antioxidant enzymes (manganese‐ and copper‐zinc‐containing superoxide dismutases, catalast and glutathione peroxidase) limit cell injury induced by reactive oxygen species. The purpose of the study was to determine whether human oral squamous cell carcinomas have altered antioxidant enzyme levels. This study is the first to undertake this task in human oral mucosa and squamous cell carcinoma.Methods:Semiquantitative immunohistochemistry was used to examine 26 archived oral squamous cell carcinoma biopsies. Fourteen well‐differentiated and 12 poorly differentiated tumors were examined, as were 12 specimens of oral mucosa. All sections were reviewed by two oral and maxillofacial pathologists, and image analysis of the immunostained sections was performed using NIH Image. Antioxidant enzyme staining intensities were compared in the different groups by Duncan's multiple range test.Results:In general, mucosal basal cells displayed lower antioxidant enzyme levels than spinous cells, and primary tumor cells displayed lower antioxidant enzyme staining intensities than did their normal cell counterparts. Moreover, poorly differentiated tumor cells showed lower antioxidant enzyme staining intensities than well‐differentiated tumor cells. Manganese‐containing superoxide dismutase staining intensities were, however, higher in well‐differentiated oral squamous cell carcinomas than their normal cells of origin.Conclusions:Detection of antioxidant enzymes may be a useful future marker in the molecular diagnosis of the oral cancer. Moreover, it may be possible to not only monitor the effectiveness of chemopreventitive and therapeutic strategies in oral cancer using these enzymes, but to monitor tumor recurrence.