Effect of polyions, Ca++, and enzymes on penetration of cultured cells by Eimeria meleagrimitis sporozoites.
Effect of polyions, Ca++, and enzymes on penetration of cultured cells by Eimeria meleagrimitis sporozoites.
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聚离子、Ca 和酶对艾美耳球虫子孢子渗透培养细胞的影响。
DOI：
10.2307/3280754
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
P. Augustine
中科院分区：
文献类型：
--
作者：
P. Augustine
用四种阳离子、两种多胺离子或两种蛋白水解酶处理来自火鸡肾脏的细胞培养物，然后接种艾美耳球虫孢子。孵育20分钟后，用三种阳离子（聚-L-组氨酸、聚-L-赖氨酸或Ca++）或胰凝乳蛋白酶处理的培养物比未处理的对照培养物含有显著更少的细胞内子孢子。聚-L-组氨酸或胰凝乳蛋白酶浓度的增加产生的子孢子数量线性下降。相比之下，在2小时孵育后，处理的和对照培养物含有相同数量的细胞内子孢子。用第四种阳离子（DEAE-葡聚糖）、两种聚阴离子（肝素或硫酸葡聚糖）或胰蛋白酶处理细胞培养物，在孵育20分钟或2小时后，均未显著影响子孢子的数量。用聚-L-组氨酸、Ca++或胰凝乳蛋白酶预处理20分钟，然后接种到未处理的培养物中的子孢子在细胞内被发现与未处理的子孢子的数量相同。显然，在处理的培养物中细胞内子孢子数量的减少是由处理物质和宿主细胞之间的相互作用引起的，而不是由寄生虫的固定引起的。
Cell cultures from kidneys of turkeys were treated with four cations, two polyamions, or two proteolytic enzymes and then inoculated with Eimeria meleagrimitis sporzoites. After a 20-min incubation, cultures treated with three of the cations (poly-L-histidine, poly-L-lysine, or Ca++) or with chymotrypsin contained significantly fewer intracellular sporozoites than did the untreated control cultures. Increases in the concentration of poly-L-histidine or chymotrypsin produced linear decreases in the numbers of sporozoites. In contrast, after a 2-hr incubation, treated and control cultures contained the same numbers of intracellular sporozoites. Treatment of cell cultures with the fourth cation (DEAE-dextran), the two polyanions (heparin or dextran sulfate), or trypsin did not significantly affect the numbers of sporozoites after either 20-min or 2-hr incubation. Sporozoites that were pretreated with poly-L-histidine, Ca++, or chymotrypsin for 20 min and then inoculated into untreated cultures were found intracellularly in the same numbers as were untreated sporozoites. Apparently, the reduced number of intracellular sporozoites in treated cultures resulted from interactions between the treatment substances and the host cells, and was not caused by immobilization of the parasite.