Prouroguanylin overproduction and localization in the intestine of zinc-deficient rats.
Prouroguanylin overproduction and localization in the intestine of zinc-deficient rats.
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DOI：
10.1093/jn/130.11.2726
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发表时间：
2000-11
期刊：
影响因子：
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通讯作者：
L. Cui;R. Blanchard;Leah M. Coy;Robert J. Cousins
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文献类型：
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作者：
L. Cui;R. Blanchard;Leah M. Coy;Robert J. Cousins
在缺锌期间，大鼠小肠中尿鸟苷素前体mRNA的上调的鉴定提供了肠道激素尿鸟苷素的产生与可能伴随缺锌的腹泻之间的潜在机制联系。在目前的研究中，原位杂交表明，前prourouroguanylin mRNA表达细胞的数量显着高于缺锌大鼠比锌充足的大鼠。免疫组织化学研究，与uroguanylin肽亲和纯化的抗体，表明免疫反应性定位于锌充足的大鼠的十二指肠和空肠的绒毛的尖端。而缺锌组阳性细胞散在分布于绒毛组织中。一个子集的细胞，也许肠嗜铬细胞，表现出主要的染色，而没有发现特定的染色杯状细胞或淋巴细胞的固有层。蛋白质印迹法证实，饲料锌缺乏可提高十二指肠和空肠中尿鸟苷素原的表达。这些结果表明，膳食锌缺乏上调肠细胞中的尿鸟苷素原，这与尿鸟苷素在人类锌缺乏症中观察到的腹泻病因学中的作用一致。
Identification of the upregulation of preprouroguanylin mRNA in the rat small intestine during zinc deficiency provides a potential mechanistic link between production of the intestinal hormone uroguanylin and the diarrhea that may accompany zinc deficiency. In the current study, in situ hybridization demonstrated that the number of preprouroguanylin mRNA-expressing cells was significantly higher in zinc-deficient rats than in zinc-adequate rats. Immunohistochemical studies, with a uroguanylin peptide affinity-purified antibody, demonstrated that immunoreactivity was localized to the tips of villi of the duodenum and jejunum in zinc-adequate rats. However, positive cells were scattered throughout the villus of zinc-deficient rats. A subset of cells, perhaps enterochromaffin cells, exhibited the predominant staining, whereas no specific staining was found in goblet cells or lymphocytes of the lamina propria. Western blotting demonstrated that the expression of prouroguanylin in both duodenum and jejunum was elevated by dietary zinc depletion. These results show that dietary zinc deficiency upregulates prouroguanylin in intestinal cells, which is consistent with a role for uroguanylin in the etiology of diarrhea observed in human zinc deficiency.