Nocardia arthritidis sp nov., a new pathogen isolated from a patient with rheumatoid arthritis in Japan
Nocardia arthritidis sp nov., a new pathogen isolated from a patient with rheumatoid arthritis in Japan
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DOI：
10.1128/jcm.42.6.2366-2371.2004
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发表时间：
2004-06-01
影响因子：
9.4
通讯作者：
Mikami, Y
中科院分区：
文献类型：
--
作者：
Kageyama, A;Torikoe, K;Mikami, Y
从类风湿性关节炎患者左大腿肿胀处的痰液和炎性分泌物中分离出两种具有不同药物敏感性的不同细菌菌株。这两种细菌菌株被暂时分配到属诺卡氏菌的基础上，其形态和化学分类学特征，并进一步研究，以建立其分类地位。其中1株（IFM 10034）经生理生化鉴定为鼻疽诺卡氏菌（Nocardia farcinica）。另一株被命名为诺卡氏菌属菌株IFM 10035（T），显示出独特的表型特性模式，将其与已建立的诺卡氏菌属物种的其他代表区分开来。通过对IFM 10035（T）菌株16 S rRNA基因序列的比较分析，发现IFM 10035（T）菌株与北京诺卡氏菌（Nocardia beijingensis）有很近的亲缘关系。然而，DNA-DNA相关性的测定表明，诺卡氏菌IFM 10035（T）菌株可以从诺卡氏菌中分离出来。北京草结合基因型和表型数据表明，该细菌值得描述为一个新的诺卡氏菌种。新物种被命名为关节炎诺卡氏菌，模式菌株是IFM 10035（T）（NBRC 100137（T），JCM 12120（T），DSM 44731（T））。目前的研究表明，诺卡氏菌感染可以由多种细菌引起。
Two different bacterial strains with different drug susceptibilities were isolated from the sputum and an inflammatory discharge from a swelling in the left thigh of a patient with rheumatoid arthritis. Both bacterial strains were provisionally assigned to the genus Nocardia on the basis of their morphological and chemotaxonomic characteristics and were further studied in order to establish their taxonomic status. One strain (IFM 10034) was identified as Nocardia farcinica on the basis of its physiological characteristics. The other strain, which was designated Nocardia sp. strain IFM 10035(T), revealed a unique pattern of phenotypic properties that distinguished it from other representatives of established Nocardia species. Comparative 16S rRNA gene sequence studies of Nocardia sp. strain IFM 10035(T) also showed that the bacterium was closely related to the species Nocardia beijingensis. Determination of DNA-DNA relatedness, however, indicated that Nocardia sp. strain IFM 10035(T) could be delineated from N. beijingensis. The genotypic and phenotypic data combined indicated that the bacterium merits description as a new Nocardia species. The name proposed for the new species is Nocardia arthritidis sp. nov., the type strain being IFM 10035(T) (NBRC 100137(T), JCM 12120(T), DSM44731(T)). The present study suggests that Nocardia infections can be caused by multiple species of the bacterium.