Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion
Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion
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DOI：
10.1016/j.bpj.2016.04.026
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发表时间：
2016-05-24
影响因子：
3.4
通讯作者：
Janshoff, Andreas
中科院分区：
文献类型：
--
作者：
Savic, Filip;Kliesch, Torben-Tobias;Janshoff, Andreas
脂膜的融合是生物学中的一个关键过程。它使细胞和细胞器能够与周围环境交换分子，否则这些分子无法穿过膜屏障。为了研究这种复杂的过程，我们使用简化的人工模型系统，即，基于膜包被玻璃球的光学融合测定。我们提出了一种技术来分析在一个大的粒子系综膜膜相互作用。通过研究光漂白实验后荧光恢复的扩散脂质动力学，获得关于完全融合的膜的融合柄的几何形状的详细信息。小的接触区是穿过熔合点的颗粒交换的强烈障碍。借助于计算机模拟，融合和单膜包衣珠的荧光恢复后光漂白恢复时间使我们能够估计两个膜包衣珠之间的接触区的大小。最小化分层和弯曲能量导致接近几何允许的最小角度。
The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.e., an optical fusion assay based on membrane-coated glass spheres. We present a technique to analyze membrane-membrane interactions in a large ensemble of particles. Detailed information on the geometry of the fusion stalk of fully fused membranes is obtained by studying the diffusional lipid dynamics with fluorescence recovery after photo-bleaching experiments. A small contact zone is a strong obstruction for the particle exchange across the fusion spot. With the aid of computer simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-membrane-coated beads allow us to estimate the size of the contact zones between two membrane-coated beads. Minimizing delamination and bending energy leads to minimal angles close to those geometrically allowed.