Identification of a herpes simplex virus 1 glycoprotein gene within a gene cluster dispensable for growth in cell culture.
Identification of a herpes simplex virus 1 glycoprotein gene within a gene cluster dispensable for growth in cell culture.
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DOI：
10.1073/pnas.84.12.4303
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发表时间：
1987-06
影响因子：
11.1
通讯作者：
R. Longnecker;S. Chatterjee;R. Whitley;B. Roizman
中科院分区：
文献类型：
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作者：
R. Longnecker;S. Chatterjee;R. Whitley;B. Roizman
单纯疱疹病毒1型的基因组由两个部分组成，L和S，每个部分都含有独特的序列，两侧是反向重复序列。目前和早期的研究表明，S组分的独特序列中所含的12个开放阅读框中的11个可以被删除，并在细胞培养中被抑制生长。对一种在开放阅读框US 7中含有缺失的重组病毒的分析允许鉴定对该基因产物具有特异性的单克隆抗体。由该基因编码的蛋白质在变性聚丙烯酰胺凝胶中的预测翻译分子量为41，366，表观分子量约为65，000。在抑制浓度衣霉素存在下由细胞合成的蛋白质的电泳迁移率比在未处理的感染细胞的裂解物中积累的蛋白质的电泳迁移率快。我们的结论是US 7的产物是糖蛋白的N-连接糖基化，我们将其命名为糖蛋白I。这些研究表明，S组分的独特序列编码四种糖蛋白（G、D、I和E），其中至少三种（G、I和E）在灵长类细胞的连续系中生长。
The genome of herpes simplex virus 1 consists of two components, L and S, each containing unique sequences flanked by inverted repeats. Current and earlier studies have shown that 11 of the 12 open reading frames contained in the unique sequences of the S component can be deleted and are dispensable for growth in cell culture. Analyses of one recombinant virus containing a deletion in the open reading frame US7 permitted the identification of a monoclonal antibody specific for the product of this gene. The protein encoded by this gene has a predicted translated molecular weight of 41,366 and an apparent molecular weight of approximately 65,000 in denaturing polyacrylamide gels. The electrophoretic mobility of the protein synthesized by cells in the presence of inhibitory concentrations of tunicamycin is faster than that of the protein accumulating in lysates of untreated infected cells. We conclude that the product of US7 is glycoprotein subject to N-linked glycosylation, and we have designated it glycoprotein I. These studies indicate that the unique sequences of the S component encode four glycoproteins (G, D, I, and E) of which at least three (G, I, and E) are dispensable for growth in continuous lines of primate cells.