Integrating optics and microfluidics for time-correlated single-photon counting in lab-on-a-chip devices
Integrating optics and microfluidics for time-correlated single-photon counting in lab-on-a-chip devices
复制标题
集成光学和微流体，在芯片实验室设备中进行时间相关的单光子计数
DOI：
10.1063/1.2772175
复制
发表时间：
2007
影响因子：
4
通讯作者：
J. Cooper
中科院分区：
文献类型：
--
作者：
A. Cleary;A. Glidle;P. Laybourn;S. García;S. Pellegrini;C. Helfter;G. Buller;J. Aitchison;J. Cooper
作者描述了低损坏光波导与实验室芯片结构的整合，以产生一个集成的光学微流体平台，用于荧光分子的时间相关的单光子计数。使用硅上的平面二氧化硅的电子束致密化来制造波导，从而消除了沉积上层二氧化硅层的任何要求。将微流体通道直接通过波导干燥，并使用聚（二甲基硅氧烷）垫圈密封设备。荧光团尼罗河蓝色的时间分辨荧光寿命测量被用作模型系统，以证明微流体设备的运行，染料浓度低至1.5nm（等效于<6000molecules）。
The authors describe the integration of low-loss optical waveguides with lab-on-a-chip structures to produce an integrated optical-microfluidic platform for time-correlated single-photon counting of fluorescent molecules. Waveguides were fabricated using electron beam densification of planar silica on silicon, eliminating any requirement for depositing upper cladding silica layers. Microfluidic channels were dry etched directly through the waveguides and the device was sealed using a poly(dimethylsiloxane) gasket. Time-resolved fluorescence lifetime measurements of the fluorophore nile blue were used as a model system to demonstrate the operation of the microfluidic device, with dye concentrations as low as 1.5nM (equivalent to <6000molecules) being measured.