Evaluation of the osteogenesis and angiogenesis effects of erythropoietin and the efficacy of deproteinized bovine bone/recombinant human erythropoietin scaffold on bone defect repair
Evaluation of the osteogenesis and angiogenesis effects of erythropoietin and the efficacy of deproteinized bovine bone/recombinant human erythropoietin scaffold on bone defect repair
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促红细胞生成素的成骨和血管生成作用评价及脱蛋白牛骨/重组人促红细胞支架修复骨缺损的功效
DOI：
10.1007/s10856-016-5714-5
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发表时间：
2016-06-01
影响因子：
3.7
通讯作者：
Kang, Pengde
中科院分区：
文献类型：
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作者：
Li, Donghai;Deng, Liqing;Kang, Pengde
促红细胞生成素(EPO)可促进血管生成，并可能在骨再生中发挥作用。本研究旨在评价促红细胞生成素(EPO)的成骨和血管生成作用，以及脱蛋白牛骨/重组人EPO支架修复骨缺损的效果。选用健康成年山羊24只，建立山羊缺损区模型，随机分为4组。分别植入DBB/rhEPO支架(A组)、DBB多孔支架(B组)、自体松质骨移植(C组)和空白对照(D组)。术后4、8、12周分别对动物进行放射学和组织学检查。结果显示，A组的愈合情况明显好于B组(P<；P<；P<0.05)。A组4周、8周灰度值低于C组(P<0.05)，12周时差异无统计学意义(P>；P>0.05)。根据组织学切片计算新生骨面积，结果表明，A组在4、8、12周时的新骨量均较B组明显增加(P<0.05)，但低于C组(P>；0.05)。免疫组织化学和实时定量聚合酶链式反应显示，12周时A组血管内皮生长因子的表达明显高于B组(P<0.05)，4周和8周时也好于C组(P<0.05)，但12周时差异无统计学意义(P&>0.05)。因此，EPO具有明显的骨形成和血管生成作用，并具有促进骨缺损修复的能力。值得进一步研究。
Erythropoietin (EPO) could promote the angiogenesis and may also play a role in bone regeneration. This study was conducted to evaluate the osteogenesis and angiogenesis effects of EPO and the efficacy of deproteinized bovine bone/recombinant human EPO scaffold on bone defect repair. Twenty-four healthy adult goats were chosen to build goat defects model and randomly divided into four groups. The goats were treated with DBB/rhEPO scaffolds (group A), porous DBB scaffolds (group B), autogenous cancellous bone graft (group C), and nothing (group D). Animals were evaluated with radiological and histological methods at 4, 8 and 12 weeks after surgery. The grey value of radiographs was used to evaluate the healing of the defects and the outcome revealed that the group A had a better outcome of defect healing compared with group B (P< 0.05). However, the grey values in group A were lower than group C at week 4 and week 8 (P< 0.05), but at week 12 their difference had no statistical significance (P> 0.05). The newly formed bone area was calculated from histological sections and the results demonstrated that the amount of new bone in group A increased significantly compared with that in group B (P< 0.05) but was inferior to that in group C (P> 0.05) at 4, 8, 12 weeks respectively. In addition, the expression of vascular endothelial growth factor (VEGF) by immunohistochemical testing and real-time polymerase chain reaction at 12 weeks in group A was significantly higher than that in group B (P< 0.05), and also better than that in group C at week 4 and week 8 (P< 0.05), but at week 12 their difference had no statistical significance (P> 0.05). Therefore, EPO has significant effects on bone formation and angiogenesis, and has capacity to promote the repair of bone defects. It is worthy of being recommended to further studies.