Effects of atmospheric pressure plasma jet on the physicochemical, functional, and antioxidant properties of flaxseed protein
Effects of atmospheric pressure plasma jet on the physicochemical, functional, and antioxidant properties of flaxseed protein
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大气压等离子体射流对亚麻籽蛋白理化、功能和抗氧化特性的影响
DOI：
10.1111/1750-3841.15184
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发表时间：
2020
影响因子：
3.9
通讯作者：
Shen Ruiling
中科院分区：
文献类型：
--
作者：
Yu Xiao;Huang Shasha;Nie Chengzhen;Deng Qianchun;Zhai Yafei;Shen Ruiling
摘要本研究旨在探讨常压等离子体射流（APPJ）处理（0 ~ 240 s）对亚麻籽蛋白理化性质、功能性质和抗氧化性质的影响。结果表明，APPJ处理后pH值持续下降，240 s后pH值最低为3.45 ± 0.15（-61.7%，P < 0.05）; APPJ处理15 s后，亚麻籽蛋白的相对溶解度显著下降（-43.1%，P < 0.05），同时亚麻籽蛋白在水溶液中的平均粒径显著增加（+157%，P < 0.05）。随着APPJ暴露时间的延长，样品表面疏水性和二硫键含量逐渐增加。经APPJ处理后，亚麻籽蛋白质的起泡性、乳化性和体外抗氧化性均得到显著改善，这与蛋白质空间构象的变化、蛋白质轻度氧化以及天然蛋白质-酚类复合物中酚酸和黄酮类化合物的释放有关。我们的研究结果表明，APPJ可以被认为是一种有效的策略，以提高亚麻籽蛋白的功能性和抗氧化活性。实际应用我们已经评估了APPJ处理对亚麻籽蛋白的物理化学，功能和抗氧化特性的影响，这有助于定制具有最佳技术功能和抗氧化活性的亚麻籽蛋白作为潜在的纳米载体。
AbstractThe aim of this study was to explore the effect of atmospheric pressure plasma jet (APPJ) on the physicochemical, functional, and antioxidant properties of flaxseed protein following APPJ treatment (0 to 240 s). The results showed that the pH value continuously dropped with the minimum value of 3.45 ± 0.15 after 240 s of APPJ treatment (–61.7%,P< 0.05). The relative protein solubility significantly declined after 15 s of APPJ treatment (–43.1%,P< 0.05), which was accompanied by the evident increase in mean particle size of flaxseed protein in aqueous solution (+157%,P< 0.05). Moreover, the surface hydrophobicity and contents of disulfide bonds gradually raised when the APPJ exposure time extended from 30 to 240 s. Notably, the foaming, emulsifying, andin vitroantioxidant properties of flaxseed protein were significantly improved following short time of APPJ treatment (5 to 15 s), which was paralleled with the changes of spatial conformation, mild protein oxidation, as well as the release of phenolic acids and flavonoids from naturally occurring protein–phenolic complex. Our findings elucidated that APPJ may be considered as an effective strategy to improve the functionality and antioxidant activities of flaxseed protein.Practical ApplicationWe had evaluated the effect of APPJ treatment on the physicochemical, functional, and antioxidant properties of flaxseed protein, which was conducive to tailor flaxseed protein with the optimal techno‐functionality and antioxidant activities as a potential nano‐delivery vehicle.