Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model
Influence of cell and collagen concentration on the cell-matrix mechanical relationship in a corneal stroma wound healing model
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DOI：
10.1016/j.exer.2010.07.013
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发表时间：
2010-11-01
影响因子：
3.4
通讯作者：
Yang, Ying
中科院分区：
文献类型：
--
作者：
Ahearne, Mark;Wilson, Samantha L.;Yang, Ying
在25天的培养期间，检查了不同胶原和细胞浓度对角膜基质伤口愈合模型的机械和重塑行为的影响，所述角膜基质伤口愈合模型由接种有人角膜成纤维细胞的胶原水凝胶组成。将人角膜成纤维细胞以每个水凝胶1 × 10（5）、3 × 10（5）或5 × 10（5）个细胞接种，并检查2.5 mg/ml、3.5 mg/ml或4.5 mg/ml的胶原蛋白浓度。两个非破坏性的技术，球形压痕和光学相干断层扫描，分别用于测量的弹性模量和尺寸的变化，在几个时间点在整个培养期间。水凝胶的弹性模量在25天内连续增加。与其他水凝胶相比，具有较高初始细胞接种密度和较低初始胶原蛋白浓度的水凝胶的弹性模量增加更快，并且在培养期结束时具有更高的弹性模量。应用数学方程精确拟合弹性模量随时间的变化。本研究证明了一种稳健的体外技术，能够在长时间培养期间监测不同参数对角膜基质模型中细胞-基质机械关系的影响，并增强了我们对角膜伤口愈合过程的理解。(C)2010爱思唯尔有限公司保留所有权利。
The effect of different collagen and cell concentrations on the mechanical and remodeling behaviors of corneal stroma wound healing models consisting of collagen hydrogels seeded with human corneal fibroblasts during a 25 day culture period were examined. Human corneal fibroblasts were seeded at 1 x 10(5), 3 x 10(5) or 5 x 10(5) cells per hydrogel, and collagen concentrations of 2.5 mg/ml, 3.5 mg/ml or 4.5 mg/ml were examined. Two non-destructive techniques, spherical indentation and optical coherence tomography, were used to measure the elastic modulus and dimensional changes respectively at several time-points over the culture period. The elastic modulus of the hydrogels increased continuously over 25 days. Hydrogels with higher initial cell seeding densities and lower initial collagen concentrations were found to increase in elastic modulus faster and possessed a higher elastic modulus by the end of the culture period when compared to the other hydrogels. A mathematical equation was applied to accurately fit the change in elastic modulus over time. This study demonstrates a robust in vitro technique able to monitor the effect of different parameters on the cell-matrix mechanical relationship in a corneal stroma model during prolonged culture periods and enhances our understanding on corneal wound healing processes. (C) 2010 Elsevier Ltd. All rights reserved.