Evidence of a novel silencing effect on transgenes in the Arabidopsis thaliana sperm cell
Evidence of a novel silencing effect on transgenes in the Arabidopsis thaliana sperm cell
复制标题
拟南芥精子细胞中转基因的新型沉默效应的证据
DOI：
10.1093/plcell/koad219
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kawashima, Tomokazu
中科院分区：
文献类型：
--
作者：
Ohnishi, Yukinosuke;Kawashima, Tomokazu
我们在拟南芥（arabidopsis thalianasperm）细胞中发现了意外的转基因沉默；转基因编码的蛋白没有特定的细胞内定位（细胞质蛋白）被转录或转录后沉默。标记有荧光蛋白基因的细胞质蛋白转基因mRNA显著减少，导致精子细胞中检测不到荧光蛋白信号。在精子细胞中沉默细胞质蛋白转基因不影响其内源同源基因的表达，也不影响编码细胞内靶向定位蛋白的共转化转基因的表达。在包括DNA甲基化在内的主要基因沉默机制的突变体中，精子细胞中的转基因沉默持续存在。精子细胞中出现的难以理解但真实存在的转基因沉默表型可能会误导对植物繁殖实验结果的解释，本评论呼吁注意这一风险，并强调了这种新型细胞质蛋白转基因沉默的细节。
We encountered unexpected transgene silencing inArabidopsis thalianasperm cells; transgenes encoding proteins with no specific intracellular localization (cytoplasmic proteins) were silenced transcriptionally or posttranscriptionally. The mRNA of cytoplasmic protein transgenes tagged with a fluorescent protein gene was significantly reduced, resulting in undetectable fluorescent protein signals in the sperm cell. Silencing of the cytoplasmic protein transgenes in the sperm cell did not affect the expression of either its endogenous homologous genes or cotransformed transgenes encoding a protein with targeted intracellular localization. This transgene silencing in the sperm cell persisted in mutants of the major gene silencing machinery including DNA methylation. The incomprehensible, yet real, transgene silencing phenotypes occurring in the sperm cell could mislead the interpretation of experimental results in plant reproduction, and this Commentary calls attention to that risk and highlights details of this novel cytoplasmic protein transgene silencing.