Mice without myoglobin
Mice without myoglobin
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DOI：
10.1038/27681
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发表时间：
1998-10-29
期刊：
影响因子：
64.8
通讯作者：
Williams, RS
中科院分区：
文献类型：
--
作者：
Garry, DJ;Ordway, GA;Williams, RS
肌红蛋白是一种在脊椎动物的心脏和氧化骨骼肌纤维中表达的细胞内血液蛋白，它与分子氧结合，可能促进氧从红细胞到线粒体的运输，从而在高生理需求时期维持细胞呼吸(1-10)。然而，我们在这里展示了，通过基因敲除技术产生的没有肌红蛋白的小鼠是有生育能力的，表现出正常的运动能力和对低氧水平(低氧)的正常呼吸反应。这些动物的心脏和比目鱼肌脱色，但在一系列工作条件和氧气供应的体外肌肉表现的标准分析中功能正常。这些数据表明，在陆地哺乳动物中，肌红蛋白不需要满足怀孕或运动的代谢要求，并对工作肌肉的氧气运输和代谢调节提出了新的问题。
Myoglobin, an intracellular haemoprotein expressed in the heart and oxidative skeletal myofibres of vertebrates, binds molecular oxygen and may facilitate oxygen transport from erythrocytes to mitochondria, thereby maintaining cellular respiration during periods of high physiological demand(1-10). Here we show, however, that mice without myoglobin, generated by gene-knockout techhnology, are fertile and exhibit normal exercise capacity and a normal ventilatory response to low oxygen levels (hypoxia). Heart and soleus muscles from these animals are depigmented, but function normally in standard assays of muscle performance in vitro across a range of work conditions and oxygen availability. These data show that myoglobin is not required to meet the metabolic requirements of pregnancy or exercise in a terrestrial mammal, and raise new questions about oxygen transport and metabolic regulation in working muscles.