A new micro-fluid chip calorimeter for biochemical applications
A new micro-fluid chip calorimeter for biochemical applications
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DOI：
10.1016/j.tca.2005.07.011
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发表时间：
2006-06-15
影响因子：
3.5
通讯作者：
Krügel, M
中科院分区：
文献类型：
--
作者：
Lerchner, J;Wolf, A;Krügel, M
基于MEMS技术开发的新型热电堆热功率传感器，构建了一种小型化的流式量热计。热功率传感器由带有薄膜铋/锑热电堆的硅片和PMMA反应室组成。为了确保高信号分辨率，热功率传感器安装在高精度恒温器内，温度稳定性小于100 μ k。根据液体的导热系数，所选反应室的高度和体积流量，量热计的热功率灵敏度为4-7 V W-1。对于小于5 μ l min（-1）的体积流量，可以获得小于50 nW的检测极限。该量热计的一个重要优点是样品消耗量低。对于特殊应用，一个测量脉冲所需的样品不超过20 μ l。(c) 2005 Elsevier B.V.版权所有。
Based on a new thermopile heat power sensor, which was developed in MEMS technology, a miniaturized flow-through calorimeter was constructed. The heat power sensor consists of a silicon chip with a thin film BiSb/Sb thermopile and a PMMA reaction chamber. To ensure high signal resolution the heat power sensor is mounted inside a high-precision thermostat, which has a temperature stability of less than 100 mu K. The heat power sensitivity of the calorimeter is 4-7 V W-1 depending on the thermal conductivity of the liquid, the height of the chosen reaction chamber and the volume flow rate. A limit of detection of less than 50 nW can be obtained for volume flows lower than 5 mu l min(-1). An important advantage is the low sample consumption of the calorimeter. For special applications the sample need for one measurement pulse does not exceed 20 mu l. (c) 2005 Elsevier B.V. All rights reserved.