DETERMINATION OF CHOLESTEROL OXIDATION-PRODUCTS IN HUMAN PLASMA BY ISOTOPE-DILUTION MASS-SPECTROMETRY
DETERMINATION OF CHOLESTEROL OXIDATION-PRODUCTS IN HUMAN PLASMA BY ISOTOPE-DILUTION MASS-SPECTROMETRY
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DOI：
10.1006/abio.1995.1110
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发表时间：
1995-02-10
影响因子：
2.9
通讯作者：
DICZFALUSY, U
中科院分区：
文献类型：
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作者：
DZELETOVIC, S;BREUER, O;DICZFALUSY, U
建立了同位素稀释-质谱法测定血浆中9种胆固醇氧化产物的方法。测定的胆固醇氧化产物为胆甾-5-烯-3 β，7 α-二醇、胆甾-5-烯-3 β，7 β-二醇（7（分别为α-和7 β-羟基胆固醇），3 β-羟基胆甾-5-烯-7-酮（7-氧代胆固醇），5，6 α-环氧-5 α-胆甾烷-3 β-醇（胆固醇-5 α，6 α-环氧化物），5，6 β-环氧-5 β-胆甾烷-3 β-醇（胆固醇-5 β，6 β-环氧化物），胆甾烷-3 β，5 α，6 β-三醇，胆甾-5-烯-3 β，24-二醇胆甾-5-烯-3 β，25-二醇（25-羟基胆固醇）和胆甾-5-烯-3 β，27-二醇（27-羟基胆固醇）。除了5 β，6 β-环氧胆固醇外，合成了每种胆固醇氧化产物的相应氘标记内标物（含有3至6个氘原子），5 β，6 β-环氧胆固醇使用5 α，6 β-环氧胆固醇的内标物测定。通过新方法分析了31名健康志愿者的血浆，27-，24-和7 β-羟基胆固醇是最丰富的胆固醇氧化产物（平均值分别为154，64和43 ng/ml）。测定的其他氧化固醇的浓度低于30 ng/ml。雄性动物血浆中的27-羟基胆固醇浓度高于雌性动物。5，6-氧化产物主要以未酯化形式存在，而其它氧化产物主要以酯化形式存在。(C)出版社：Academic Press
A method based on isotope dilution-mass spectrometry was developed for the determination of nine cholesterol oxidation products in human plasma. The cholesterol oxidation products determined were cholest-5-ene-3 beta,7 alpha-diol, cholest-5-ene-3 beta,7 beta diol (7(alpha- and 7 beta-hydroxycholesterol, respectively), 3 beta-hydroxycholest-5-en-7-one (7-oxocholesterol),5,6 alpha-epoxy-5 alpha-cholestan-3 beta-ol (cholesterol-5 alpha,6 alpha-epoxide), 5,6 beta-epoxy- 5 beta- cholestan-3 beta- ol (cholesterol-5 beta,6 beta- epoxide), cholestane-3 beta,5 alpha,6 beta-triol, cholest-5-ene-3 beta,24-diol (24-hydroxycholesterol), cholest-5-ene-3 beta,25-diol (25-hydroxycholesterol), and cholest-5-ene-3 beta,27-diol (27-hydroxycholesterol). A corresponding deuterium-labeled internal standard, containing 3 to 6 deuterium atoms, was synthesized for each cholesterol oxidation product except 5 beta,6 beta-epoxycholesterol which was determined using the internal standard for 5 alpha,6 beta-epoxycholesterol. Plasma from 31 healthy volunteers was analyzed by the new method and 27-, 24-, and 7 beta-hydroxycholesterol were the most abundant cholesterol oxidation products (mean values 154, 64, and 43 ng/ml, respectively). The other oxysterols determined were present in concentrations lower than 30 ng/ml. Males had higher 27-hydroxycholesterol concentrations in plasma than females. The 5,6-oxygenated products were present mainly unesterified while the other oxidation products were mostly in esterified form. (C) 1995 Academic Press, Inc.