Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice
Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice
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DOI：
10.4062/biomolther.2013.071
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发表时间：
2013-11-30
影响因子：
3.7
通讯作者：
Kim, Sun Yeou
中科院分区：
文献类型：
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作者：
Park, Hye Min;Kim, Hye Jin;Kim, Sun Yeou
紫外线(UV)辐射是导致人体皮肤急性和慢性反应的主要环境因素。紫外线照射会导致皱纹形成、DNA损伤和产生活性氧(ROS)。大多数与紫外线照射皮肤相关的皮肤生理学和药理学的机制研究都集中在蛋白质及其相关基因表达或单靶向小分子上。本研究采用实时直接分析(DART)飞行时间质谱仪(TOF-MS)和多元分析相结合的方法，对UVB照射和局部应用维甲酸(RP，5%)治疗无毛小鼠后皮肤代谢产物的变化进行了鉴定和分析。在负离子模式下，DART离子源成功地电离了棕榈油酸和亚麻酸等多种脂肪酸。从阳性模式下测得的DART-TOF-MS指纹图谱中，三组均出现了显著的胆固醇脱水离子峰(m/z：369，M+H-H2O)。在阳性模式下，经多元正交偏最小二乘判别分析(OPLS-DA)，三组间的区分度明显高于阴性模式。DART-TOF-MS可以电离活组织中的各种小有机分子，是一种有效的替代分析工具，无需样品制备就可以从活组织中获得完整的化学指纹图谱。DART-MS测量皮肤组织的多变量分析被证明是一种在皮肤病研究中区分实验人群和寻找各种实验模型的生物标志物的有效方法。
Ultraviolet (UV) radiation is a major environmental factor that leads to acute and chronic reactions in the human skin. UV exposure induces wrinkle formation, DNA damage, and generation of reactive oxygen species (ROS). Most mechanistic studies of skin physiology and pharmacology related with UV-irradiated skin have focused on proteins and their related gene expression or single-targeted small molecules. The present study identified and analyzed the alteration of skin metabolites following UVB irradiation and topical retinyl palmitate (RP, 5%) treatment in hairless mice using direct analysis in real time (DART) time-of-flight mass spectrometry (TOF-MS) with multivariate analysis. Under the negative ion mode, the DART ion source successfully ionized various fatty acids including palmitoleic and linolenic acid. From DART-TOF-MS fingerprints measured in positive mode, the prominent dehydrated ion peak (m/z: 369, M+H-H2O) of cholesterol was characterized in all three groups. In positive mode, the discrimination among three groups was much clearer than that in negative mode by using multivariate analysis of orthogonal partial-least squares-discriminant analysis (OPLS-DA). DART-TOF-MS can ionize various small organic molecules in living tissues and is an efficient alternative analytical tool for acquiring full chemical fingerprints from living tissues without requiring sample preparation. DART-MS measurement of skin tissue with multivariate analysis proved to be a powerful method to discriminate between experimental groups and to find biomarkers for various experiment models in skin dermatological research.