Enhancement of tendon‑bone healing following rotator cuff repair using hydroxyapatite with TGFβ1.
Enhancement of tendon‑bone healing following rotator cuff repair using hydroxyapatite with TGFβ1.
复制标题
使用羟基磷灰石和 TGF1 增强肩袖修复后的腱骨愈合
DOI：
10.3892/mmr.2018.8499
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发表时间：
2018-04
影响因子：
3.4
通讯作者：
He Y
中科院分区：
文献类型：
--
作者：
You X;Shen Y;Yu W;He Y
肌腱袖撕裂修复后愈合部位纤维软骨的形成是肌腱-骨愈合领域的一个主要问题。本研究旨在通过包裹转化生长因子β1 （tgf - β1）的羟基磷灰石（HA）介入肌腱袖撕裂修复后肌腱-骨界面的愈合。采用急性肩袖修复模型，将大鼠分为三组：ⅰ)仅修复组（对照组）；ii) HA组；iii) HA-TGFβ1组。分别于术后2周、4周和8周处死。显微计算机断层扫描（CT），组织形态学分析和生物力学测试评估冈上肌腱-骨复合体。微ct图像显示，ha - tgf - β1治疗组明显有新的骨形成。组织形态学分析显示肌腱-骨界面的纤维软骨形成和胶原组织得到改善。ha - tgf - β1联合治疗可显著改善纤维软骨面积，特别是在早期时间点（2周和4周）。与对照组相比，HA和HA- tgf β1组在4周和8周时获得的载荷-失效力明显更大。与单纯手术修复相比，ha - tgf - β1增强陶瓷粉在肌腱-骨界面处可以增强愈合体，增加骨和纤维软骨的形成，改善胶原组织。局部应用HA-TGFβ1可改善肩袖修复后的肌腱-骨愈合。
The formation of fibrocartilage at the healing site following a rotator cuff tear repair is a major problem in the field of tendon-bone healing. The present study aimed to enhance the healing of the tendon-bone interface following rotator-cuff tear repair by the interposition of hydroxyapatite (HA) encapsulated with transforming growth factor β1 (TGFβ1). Using an acute rotator cuff repair model, rats were divided into three groups: i) Repair only (control); ii) HA group; and iii) HA-TGFβ1 group. Animals were sacrificed at 2, 4 and 8 weeks following surgery. Micro-computed tomography (CT), histomorphometric analyses and biomechanical tests were used to evaluate the supraspinatus tendon-bone complex. The micro-CT images revealed notable novel bone formation in the groups treated with HA-TGFβ1. The histomorphometric analyses demonstrated improved fibrocartilage formation and collagen organization at the tendon-bone interface. The HA-TGFβ1 combination significantly improved the area of fibrocartilage, particularly at early time points (2 and 4 weeks). There was a significantly greater load-to-failure force achieved in the HA and HA-TGFβ1 groups compared with the control group at 4 and 8 weeks. Augmentation of the ceramic powder with HA-TGFβ1 at the tendon-bone interface was demonstrated to strengthen the healing entheses, increase bone and fibrocartilage formation and improve collagen organization compared with surgical repair alone. Local application of HA-TGFβ1 demonstrates potential in improving tendon-bone healing following rotator cuff repair.
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影响因子：
82.9
作者：
通讯作者：
--
影响因子：
4.6
作者：
Pryce, Brian A.;Watson, Spencer S.;Schweitzer, Ronen
通讯作者：
Schweitzer, Ronen
DOI：
10.1016/j.arthro.2006.12.031
发表时间：
2007-05-01
影响因子：
4.7
作者：
Huangfu, Xiaoqiao;Zhao, Jinzhong
通讯作者：
Zhao, Jinzhong
影响因子：
2.9
作者：
Kim, H. Mike;Galatz, Leesa M.;Thomopoulos, Stavros
通讯作者：
Thomopoulos, Stavros
影响因子：
2.8
作者：
Galatz, LM;Sandell, LJ;Thomopoulos, S
通讯作者：
Thomopoulos, S