Surface modification of poly(dimethylsiloxane) by two-step plasma treatment for further grafting with chitosan-Rose Bengal photosensitizer
Surface modification of poly(dimethylsiloxane) by two-step plasma treatment for further grafting with chitosan-Rose Bengal photosensitizer
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DOI：
10.1016/j.surfcoat.2013.02.035
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发表时间：
2013-05-25
影响因子：
5.4
通讯作者：
Ciardelli, Gianluca
中科院分区：
文献类型：
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作者：
Ferreira, Ana Marina;Carmagnola, Irene;Ciardelli, Gianluca
聚二甲基硅氧烷（PDMS）由于其生物相容性和生物耐久性而在制药和医疗器械中得到应用。本研究旨在将壳聚糖-虎红（CH.RB）接枝到PDMS基质上，以降低手术应用过程中细菌感染的风险。用氩等离子体活化PDMS，并用丙烯酸官能化以引入羧基部分，用于与CH.RB氨基基团的进一步共价偶联反应。通过甲苯胺蓝-O比色法，在丙烯酸官能化的PDMS（PDMS-pAAc）表面上可接近的羧基被定量为1.9 +/- 0.1 μ g/cm（2）。在PDMS-pAAc ATR-FTIR光谱中在3100-3700、1726和1633 cm（-1）处出现ATR-FTIR吸收峰表明引入了羧基，而在CH.RB官能化PDMS（PDMS-pAAc-CH.RB）的ATR-FTIR光谱中在1651和1590 cm（-1）处的峰证明了酰胺键的形成。XPS光谱中的去卷积C1 s峰的分析证实了在每个官能化步骤的表面化学改性。表面润湿性受表面官能化的影响：PDMS是疏水性的（111度），而PDMS-pAAc和PDMS-pAAc-CH.RB是亲水性的（分别为55度和58度）。用大肠杆菌ATCC 25922和金黄色葡萄球菌ATCC 6538的增殖细胞初步研究PDMS-pAAC-CH.RB的抗菌活性。(C)2013爱思唯尔有限公司版权所有。
Polydimethylsiloxane (PDMS) finds applications in pharmaceutical and medical devices, due to its biocompatibility and biodurability. This work was aimed at grafting chitosan-Rose Bengal (CH.RB) on PDMS substrates to reduce the risks of bacterial infection during surgical application. PDMS was activated with argon plasma and functionalized with acrylic acid to introduce carboxyl moieties for further covalent coupling reaction with CH.RB amino groups. The accessible carboxylic groups on the acrylic acid functionalized PDMS (PDMS-pAAc) surface were quantified to be 1.9 +/- 0.1 mu g/cm(2) by toluidine blue-O colorimetric method. The appearance of ATR-FTIR absorption peaks at 3100-3700, 1726 and 1633 cm(-1) in the PDMS-pAAc ATR-FTIR spectrum suggested the introduction of carboxyl groups, whereas the peaks at 1651 and 1590 cm(-1) in the ATR-FTIR spectrum of CH.RB functionalized PDMS (PDMS-pAAc-CH.RB) evidenced the amide bond formation. Analysis of deconvoluted C1s peak in XPS spectra confirmed surface chemical modification at each functionalization step. Surface wettability was affected by surface functionalization: PDMS was hydrophobic (111 degrees), whereas PDMS-pAAc and PDMS-pAAc-CH.RB were hydrophilic (55 degrees and 58 degrees respectively). PDMS-pAAC-CH.RB antibacterial activity was preliminarily investigated using planktonic cells of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 6538. (C) 2013 Elsevier B.V. All rights reserved.