Microfluidics on Standard Petri Dishes for Bioscientists.
Microfluidics on Standard Petri Dishes for Bioscientists.
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为生物科学家提供的标准培养皿上的微流体。
DOI：
10.1002/smtd.202100724
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发表时间：
2021
期刊：
影响因子：
12.4
通讯作者：
Deroy C
中科院分区：
文献类型：
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作者：
Deroy C
尽管有明显的潜力，但很少有微流体设备用于生物医学实验室;给出的原因包括这些设备很少用细胞友好材料制成，并且液体难以隐藏在固体限制壁后面。回顾了一种开放的微流体方法，其中通过重塑两种液体（细胞培养基加上不混溶和生物惰性的氟碳化合物，FC 40），在标准聚苯乙烯培养皿上在几分钟内制造出几乎具有任何可想象的2D形状的水回路。然后，水相变得被流体FC 40壁限制，该流体FC 40壁通过界面力牢固地钉在皿上。这样的壁可以在任何点处用移液管刺穿，并且通过它们添加或移除液体，而流动可以使用外部泵主动地驱动或通过利用拉普拉斯压力的局部差异被动地驱动。由于细胞壁坚固，可渗透O2 + CO2，并且透明，因此细胞在培养箱中生长，并像往常一样进行显微镜监测。人们希望这种简单、方便、经济实惠的流体成形技术能为生物科学家提供一个进入微流体领域的简单入口。
Few microfluidic devices are used in biomedical labs, despite the obvious potential; reasons given include the devices are rarely made with cell‐friendly materials, and liquids are inaccessibly buried behind solid confining walls. An open microfluidic approach is reviewed in which aqueous circuits with almost any imaginable 2D shape are fabricated in minutes on standard polystyrene Petri dishes by reshaping two liquids (cell‐culture media plus an immiscible and bioinert fluorocarbon, FC40). Then, the aqueous phase becomes confined by fluid FC40 walls firmly pinned to the dish by interfacial forces. Such walls can be pierced at any point with pipets and liquids added or removed through them, while flows can be driven actively using external pumps or passively by exploiting local differences in Laplace pressure. As walls are robust, permeable to O2plus CO2, and transparent, cells are grown in incubators and monitored microscopically as usual. It is hoped that this simple, accessible, and affordable fluid‐shaping technology provides bioscientists with an easy entrée into microfluidics.